法医学杂志

法医学杂志 ›› 2008, Vol. 0 ›› Issue (6): 435-436.

• 论文 • 上一篇    下一篇

牙刷刷毛黏附口腔黏膜脱落细胞DNA的检验方法

封宇;严密;刘云;陈骏;   

  1. 重庆市公安局物证鉴定中心;川北医学院法医学系;
  • 发布日期:2008-12-25 出版日期:2008-12-28

A Method for DNA Examination on Mouth Mucosa Exfoliative Cells from Tooth-Brush Bristles

FENG YU1, YAN MI1, LIU YUN2, CHEN JUN1 (1. INSTITUTE OF FORENSIC SCIENCE, CHONGQING PUBLIC SECURITY BUREAU, CHONGQING 400042, CHINA; 2. DEPART- MENT OF FORENSIC MEDICINE, NORTH SICHUAN MEDICAL COLLEGE, NANCHONG 637000, CHINA)   

  • Online:2008-12-25 Published:2008-12-28

被引次数

5

摘要： 目的探索牙刷刷毛黏附口腔黏膜脱落细胞DNA提取有效方法。方法采用牙刷刷毛拔出法和直接涮洗法收集口腔黏膜脱落细胞,分别用Chelex-100法和DNAIQ试剂盒提取DNA进行PCR扩增及STR分型。结果牙刷刷毛拔下后收集口腔黏膜脱落细胞检测STR9个以上基因座成功率明显高于直接涮洗法,差异具统计学意义(P<0.05)。Chelex-100法和DNAIQ试剂盒提取法之间差异无统计学意义(P>0.05)。结论牙刷刷毛拔出法较直接涮洗法收集口腔黏膜脱落细胞更有效。

关键词: 法医遗传学, 提取法, 口腔黏膜, 脱落细胞, 牙刷刷毛

Abstract: Objective To establish a method for DNA examination on mouth mucosa exfoliative cells from toothbrush bristles. Methods Slough-off mouth mucosa exfoliative cells were collected by bristles extracted method and direct washing method. The DNA of exfoliative cells was extracted with Chelex-100 method and DNA IQTM kit. Then all the extracted DNA took PCR amplification and STR analysis. Results There was a significant difference between the groups with bristles extracted method and with direct washing method at the success rates of over 9 STR loci detected (P<0.05). However, there was no statistical significance between Chelex-100 method and DNA IQTM kit (P>0.05). Conclusion It is demonstrated that mouth mucosa exfoliative cells collected by bristles extracted method is more available than that with direct washing method.

Key words: forensic genetics, extraction, mouth mucosa, exfoliative cell, toothbrush-bristles