法医学杂志

法医学杂志 ›› 2009, Vol. 0 ›› Issue (3): 187-191.

• 论文 • 上一篇    下一篇

Mentype~ Argus X-8试剂盒在亲权鉴定中的应用价值

张素华;畅晶晶;李莉;   

  1. 苏州大学医学部法医学系;司法部司法鉴定科学技术研究所上海市法医学重点实验室;复旦大学上海医学院;
  • 发布日期:2009-06-25 出版日期:2009-06-28

Application of Mentype~ Argus X-8 Kit in Forensic Paternity Testing

ZHANG SU-HUA1,3,CHANG JING-JING2,3,LI-LI3(1.DEPARTMENT OF FORENSIC MEDICINE,MEDICAL COLLEGE OF SOOCHOW UNIVERSITY,SUZHOU 215123,CHINA;2.SHANGHAI MEDICAL COLLEGE,FUDAN UNIVERSITY,SHANGHAI 200032,CHINA;3.SHANGHAI KEY LABORATORY OF FORENSIC MEDICINE,INSTITUT   

  • Online:2009-06-25 Published:2009-06-28

摘要： 目的验证Mentype Argus X-8试剂盒在中国人群中的应用价值,积累该试剂盒中所包含的8个X-STR基因座在中国人群中的遗传学数据。方法应用Mentype Argus X-8试剂盒对华东地区177名汉族无关个体的血样以及104例二联体亲权鉴定案例进行X-STR分型,统计分析试剂盒中所包含的8个X-STR基因座的频率分布及遗传多态性信息。结果8个X-STR基因座在中国人群中的多态性信息量在0.4454～0.9187,其中以DXS10135和DXS10101的多态信息含量最为丰富,在亲权鉴定案例中的排除率亦最高。除DXS7132基因座外,其余7个基因座在男性及女性群体中的分布差异无统计学意义。另外,中国人群中检测到一些新的等位基因:DXS10074(等位基因15.3)、DXS10101(等位基因36)和DXS10134(等位基因37.3)。结论Mentype Argus X-8试剂盒所包含的8个基因座具有高度多态性,在特殊亲权案件的鉴定中具有重要的应用价值。

关键词: 法医遗传学, X-STR, Mentype Argus X-8试剂盒, 亲权鉴定

Abstract: Objective To evaluate the application value of Mentype Argus X-8 kit in Chinese population,and to gather the genetic data of the eight X-STR loci.Methods The X-STR of 177 blood samples of unrelated East China Han population and 104 duo paternity testing cases was typed using Mentype Argus X-8 kit.The allele frequency and genetic data of the eight loci were investigated.Results The PIC of the eight X-STR loci was between 0.445 4 and 0.918 7.Among them,DXS10135 and DXS10101 had the highest PIC and exclusion rate in paternity cases.The other seven loci showed no difference in sexrelated allele frequency except DXS7132.In addition,there were several new alleles detected including DXS10074(allele 15.3),DXS10101(allele 36) and DXS10134(allele 37.3).Conclusion Eight X-STR loci included in Mentype Argus X-8 kit are high polymorphic and valuable.

Key words: forensic genetics, X-STR, Mentype Argus X-8 kit, paternity testing