法医学杂志 ›› 2010, Vol. 26 ›› Issue (4): 257-259.DOI: 10.3969/j.issn.1004-5619.2010.04.005

• 论著 • 上一篇    下一篇

Micro-XRF法检测肺内抗酸性硅质颗粒及其在溺死诊断中的应用

胡孙林1,温锦锋1,赖文彬1,方  超2,张小婷1,王松才1,戴维列1,刘  超1   

  1. (1. 广州市刑事科学技术研究所,广东 广州 510030; 2. 中山大学 中山医学院法医学系,广东 广州 510080)
  • 发布日期:2010-08-25 出版日期:2010-08-28
  • 作者简介:胡孙林(1976—),男,浙江建德人,博士,主要从事微量物证检验和法医物证学研究;E-mail:sunlin.hu@yahoo.com.cn
  • 基金资助:

    广州市科委科技攻关项目(2007Z1-E0061)

Determination of Acid-resistant Silicic Particles in Lung by Micro-XRF and Its Application in Diagnosis of Drowning

HU SUN-LIN1, WEN JIN-FENG1, LAI WEN-BIN1, FANG CHAO2, ZHANG XIAO-TING1, WANG SONG-CAI1, DAI WEI-LIE1, LIU CHAO1   

  1. (1. Guangzhou Institute of Criminal Science and Technology, Guangzhou 510030, China; 2. Department of Forensic Medicine, Zhongshan Medical College, Sun Yat-sen University, Guangzhou 510080, China)
  • Online:2010-08-25 Published:2010-08-28

摘要: 目的 利用微束X射线荧光光谱(microbeam X-ray fluorescence,Micro-XRF)分析技术检测肺内抗酸性硅质颗粒,并探讨其在法医学溺死诊断中的应用价值。 方法 将32只大白兔随机分为溺死组(n=12)、死后入水组(n=10)和对照组(n=10),分别提取现场水样和各组肺组织。采用Micro-XRF法测定现场水样和肺组织中抗酸性硅质颗粒的面积含量。 结果 现场水样中抗酸性硅质颗粒面积含量为4.4 mm2/mL;溺死组、死后入水组、对照组肺组织中抗酸性硅质颗粒面积含量分别为(25.30±10.95) mm2/g、(1.68±0.63) mm2/g、(1.65±0.85) mm2/g;溺死组肺组织中抗酸性硅质颗粒面积含量较死后入水组、对照组差异有统计学意义。 结论 肺内抗酸性硅质颗粒面积含量可作为一种溺死诊断的指标,Micro-XRF测定法灵敏度高、分析速度快,在法医学溺死诊断中具有潜在的应用价值。

关键词: 法医病理学, 溺水, 肺, 微束X射线荧光光谱, 硅质颗粒,

Abstract: Objective Using microbeam X-ray fluorescence(Micro-XRF) analyzer for determination of acid-resistant silicic particles in lung, and to explore its potential application in diagnosis of drowning. Methods Thirty two white rabbits were divided randomly into drowning group(n=12), post-mortem immersion group (n=10) and control group (n=10). Lungs and water sample were collected for determination of area concentration of acid-resistant silicic particles using Micro-XRF method. Results The area concentration of acid-resistant silicic particles for the drowning water sample was 4.4 mm2/mL. For the lungs of drowning group, the post-mortem immersion group and the control group, the determined average values were (25.30±10.95) mm2/g, (1.68±0.63) mm2/g and (1.65±0.85) mm2/g, respectively, with a statistically significant difference between the drowning group and the other two groups. Conclusion The area concentration of acid-resistant silicic particles in lungs may be used as an indicator of drowning. The method is highly sensitive and rapid. It provides a potential application in drowning diagnosis.

Key words: forensic pathology, drowning, lung, microbeam X-ray fluorescence, silica parti, rabbits

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