法医学杂志

法医学杂志 ›› 2010, Vol. 26 ›› Issue (4): 260-265.DOI: 10.3969/j.issn.1004-5619.2010.04.006

• 论著 • 上一篇    下一篇

雷公藤甲素对大鼠下丘脑-垂体-肾上腺轴的影响

朱建华1，胡永良1，张  武1，关  伟2，黄光照3   

  1. （1. 皖南医学院 医学三系，安徽 芜湖 241002； 2. 长沙市星城地区检察院，湖南 长沙 410000； 3. 华中科技大学 同济医学院，湖北 武汉 430030）
  • 发布日期:2010-08-25 出版日期:2010-08-28
  • 作者简介:朱建华（1951—），男，安徽桐城人，副教授，硕士研究生导师，主要从事法医毒理学研究；Tel：0553-3932611
  • 基金资助:

    安徽省高校省级自然科学研究项目（2006KJ413B）

Effects of Triptolide on Hypothalamic-pituitary-adrenal Axis of Rats

ZHU JIAN-HUA1, HU YONG-LIANG1, ZHANG WU1, GUAN WEI2, HUANG GUANG-ZHAO3   

  1. （1. The Third Medical Department, Wannan Medical College, Wuhu 241002, China; 2. People’s Procuratorate of Xingcheng District, Changsha 410000, China; 3. Tongji Medical School, Huazhong University of Science and Technology, Wuhan 430030, China）
  • Online:2010-08-25 Published:2010-08-28

被引次数

21

摘要： 目的 从形态和功能两方面观察雷公藤甲素（Triptolide，TP）对大鼠下丘脑-垂体-肾上腺轴（hypothalamic-pituitary-adrenal axis，HPAA）的影响。 方法 将雄性SD大鼠随机分为3组，分别灌服2％丙二醇、丙二醇醋酸泼尼松混合溶液和丙二醇雷公藤甲素混合溶液7周，检测各组血浆皮质醇（cortisol，COR）、促肾上腺皮质激素（adrencocorticotropic hormone，ACTH）和促肾上腺皮质激素释放激素（corticotropin releasing hormone，CRH）含量；行体质量、肾上腺器官系数测定及肾上腺皮质束状带组织病理学观察；免疫组织化学染色法观察ACTH在各组垂体中的表达。 结果 （1）TP组与泼尼松组血浆COR含量低于对照组（P<0.05）；血浆ACTH与对照组比较无显著差异；TP组血浆CRH高于对照组（P<0.05）。（2）TP组与泼尼松组大鼠实验后体质量均低于对照组，肾上腺器官系数与对照组比较无差异；光镜及电镜观察均表明TP组与泼尼松组肾上腺皮质束状带变薄、萎缩，肾上腺功能低下；ACTH在TP组大鼠垂体中的表达高于对照组（P<0.05）。 结论 形态和功能两方面均提示较长期服用TP可引起大鼠肾上腺皮质萎缩、功能低下，通过负反馈调节，进而使下丘脑和垂体合成、分泌CRH和ACTH增加。

关键词: 法医毒理学, 雷公藤内酯, 下丘脑-垂体系统, 免疫组织化学, 大鼠

Abstract: Objective To observe the effects of triptolide on the hypothalamic-pituitary-adrenal axis（HPAA）of rats in light of morphological and functional changes. Methods Thirty Sprague-Dawley（SD） male rats were randomized into 3 groups and given 2％ propylene glycol, mixture of propylene glycol and prednisone acetate or compounds of propylene glycol and triptolide by gavage, respectively, for consecutive 7 weeks. Determination in the 3 groups was conducted concerning the contents of blood plasma cortisol（COR）, adrenocorticotropic hormone（ACTH）and corticotropin-releasing hormone（CRH） besides measurement of the rats’ body weight, coefficient of the adrenal gland and observation of the histopathological changes in fascicular zone of adrenal cortex. Immunohistochemical staining technique was used to detect the expression of ACTH in pituitary in the 3 groups. Results （1） The content of COR in the groups of triptolide and prednisone acetate appeared lower and serum ACTH showed no significant difference, but CRH in the group of triptolide was augmented as compared with the control group（P<0.05）. （2） The rats’ weight in the groups of triptolide and prednisone acetate was declined, and yet, the coefficient of the adrenal gland remained no significant change in comparison with the controls. HE staining and electron microscopy examination revealed thinned and constricted zona fasciculata in adrenal gland in the rats of triptolide and prednisone acetate, with hypofunction. ACTH expression in the group of triptolide was higher than that of the control group（P<0.05）. Conclusion Morphologically and functionally, the findings suggest that long-term use of triptolide may result in atrophied cortex and hypofunction of the adrenal gland, leading to augmented production and secretion of CRH and ACTH from respective hypothalamic and pituitary.

Key words: forensic toxicology, triptolide, hypothalamo-hypophyseal system, immunohistochemistry, rats

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