法医学杂志

法医学杂志 ›› 2011, Vol. 27 ›› Issue (2): 107-111.DOI: 10.3969/j.issn.1004-5619.2011.02.007

• 论著 • 上一篇    下一篇

闭合性脑损伤后血清和海马的蛋白质表达

舒清明1，李志强2，李灵芝3，杨术旺3，战  丽3，张永亮3   

  1. （1. 武警总医院 病理科，北京 100039； 2. 武警内蒙古总队医院，内蒙古 呼和浩特 010000； 3. 武警医学院，天津 300162）
  • 发布日期:2011-04-25 出版日期:2011-04-28
  • 通讯作者: 通信作者：张永亮，男，教授，博士研究生导师，主要从事颅脑损伤的分子病理学研究；E-mail：zyongliang@yahoo.com.cn
  • 作者简介:舒清明（1978—），女，四川南充人，主治医师，博士研究生，主要从事闭合性颅脑损伤的分子病理学研究
  • 基金资助:

    国家自然科学基金资助项目（30471934）

Expression of Proteins in Serum and Hippocampus after Closed Brain Injury in Rats

SHU QING-MING1, LI ZHI-QIANG2, LI LING-ZHI3, YANG SHU-WANG4, ZHAN LI4, ZHANG YONG-LIANG5   

  1. （1. Department of Pathology, General Hospital of Chinese People’s Armed Police Forces, Beijing 100039, China; 2. Inner Mongolian Corps Hospital, Chinese People’s Armed Police Forces, Huhhot 010000, China; 3. Medical College of Chinese People’s Armed Police Force, Tianjin 300162, China）
  • Online:2011-04-25 Published:2011-04-28

被引次数

7

摘要： 目的 研究大鼠脑损伤后血清和海马中蛋白质表达的差异。 方法 用雄性SD大鼠制造脑损伤模型，应用弱阳离子交换（WCX2）芯片和铜离子结合（IMAC-Cu）芯片结合表面增强激光解析电离飞行时间质谱技术，分析大鼠闭合性脑损伤后不同时间血清和海马中蛋白质表达谱的变化。 结果 WCX2芯片在血清和海马样本中分别捕获436个和364个蛋白质峰，IMAC-Cu芯片在血清和海马样本中分别捕获229个和345个蛋白质峰。WCX2芯片在血清和海马中都能捕获的蛋白质峰为10个，IMAC-Cu芯片在血清和海马中都能捕获的蛋白质峰为13个。 结论 闭合性脑损伤可引起血清和海马蛋白质表达谱发生变化，且二者存在显著差异，同种芯片在血清和海马中检测到的差异蛋白质，可能是脑损伤生物标志蛋白。

关键词: 法医遗传学, 脑损伤, 血清, 海马, 蛋白质芯片, 大鼠

Abstract: Objective To explore the difference of expression of proteins between the serum and hippocampus after brain injury in rats. Methods Male SD rats were used to establish brain injury model. The changes of proteins expression profile in serum and hippocampus at different time after brain injury were analyzed using weak cationic exchanger （WCX2） chips and immobilized metal affinity capture arrays-Cu （IMAC-Cu） chips by surface-enhanced laser desorption/ionization time-of-flight mass spectrometry. Results A total of 436 protein peaks were detected in serum and 346 protein peaks were detected in hippocampus using WCX2 chips. A total of 229 protein peaks were detected in serum and 345 protein peaks were detected in hippocampus using IMAC-Cu chips. The same 10 protein peaks were respectively detected in serum and hippocampus using WCX2 chips. The same 13 protein peaks were respectively detected in serum and hippocampus using IMAC-Cu chips. Conclusion The changes of protein expression profile in serum and hippocampus are obvious after closed brain injury and show a significant difference. The different proteins detected in serum and hippocampus using the same chip could be biochemical markers for determining brain injury.

Key words: forensic genetics, brain injury, serum, hippocampus, protein chip, rats

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