法医学杂志

法医学杂志 ›› 2012, Vol. 28 ›› Issue (6): 448-450.DOI: 10.3969/j.issn.1004-5619.2012.06.013

• 技术与应用 • 上一篇    下一篇

Expressmarker 22 STR荧光检测试剂盒的法医学应用

邹凯南1，曹  禹1，夏子芳2，郑卫国2，周怀谷1   

  1. （1. 上海市公安局物证鉴定中心 上海市现场物证重点实验室，上海 200083； 2. 无锡中德美联生物技术有限公司，江苏 无锡 214174）
  • 发布日期:2012-12-25 出版日期:2012-12-28
  • 通讯作者: 周怀谷，男，博士，主任法医师，主要从事法医DNA分析技术的应用和研究；E-mail：hgzhou803@hotmail.com
  • 作者简介:邹凯南（1983—），女，黑龙江哈尔滨人，主要从事法医DNA分析技术的应用和研究；E-mail：kitty_zkn@126.com
  • 基金资助:

    公安部重点研究计划项目（2009ZDYJSHSJ012）；上海市科学技术委员会科研计划项目（09231203600）

Forensic Application of Expressmarker 22 STR Loci Direct PCR Amplification Kit

ZOU KAI-NAN1, CAO YU1, XIA ZI-FANG2, ZHENG WEI-GUO2, ZHOU HUAI-GU1   

  1. （1. Shanghai Key Laboratory of Crime Scene Evidence, Institute of Forensic Science, Shanghai Public Security Bureau, Shanghai 200083, China; 2. AGCU Biotechnology Company, Wuxi 214174, China）
  • Online:2012-12-25 Published:2012-12-28

被引次数

2

摘要： 目的 验证Expressmarker 22 STR荧光检测试剂盒的法医学应用价值。 方法 取FTA卡、滤纸上保存的建库血样和各类涉案生物性检材1 948份，用Expressmarker 22 STR荧光检测试剂盒进行STR分型检测，同时采用SinofilerTM、Identifiler、PowerPlex 16试剂盒进行平行试验。对4个试剂盒相同基因座的分型进行比对，以确认Expressmarker 22 STR荧光检测试剂盒的灵敏度和准确性。 结果 Expressmarker 22 STR荧光检测试剂盒的检测成功率为97.79%，同一样本在相同基因座与SinofilerTM、Identifiler、PowerPlex 16试剂盒的STR分型结果相同。 结论 利用Expressmarker 22 STR荧光检测试剂盒进行STR分型，信息量大、结果准确可靠，可应用于法庭科学。

关键词: 法医遗传学, 短串联重复序列, Expressmarker 22 STR荧光检测试剂盒

Abstract: Objective To explore the application value of Expressmarker 22 STR loci direct PCR amplification kit. Methods One thousand nine hundred and forty-eight samples （including samples spotted on FTA cards, filter papers and case samples） were tested using Expressmarker 22 STR loci direct PCR amplification kit. At the same time, all were tested using SinofilerTM kit, Identifiler kit and PowerPlex 16 kit respectively for comparison. The genotypes were compared at the same STR loci among these four kits to test the sensitivity and accuracy of Expressmarker 22 STR loci direct PCR amplification kit. Results 97.79% samples were successfully typed using Expressmarker 22 STR loci direct PCR amplification kit. The genotype profiles of the same samples using Expressmarker 22 STR loci direct PCR amplification kit were consistent with SinofilerTM kit, Identifiler kit and PowerPlex 16 kit at the same STR loci. Conclusion Expressmarker 22 STR loci direct PCR amplification kit can provide huge information and accurate results and be applied to forensic DNA genotyping.

Key words: forensic genetics, STR, Expressmarker 22 STR loci direct PCR amplification kit

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