法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (2): 81-85.DOI: 10.3969/j.issn.1004-5619.2016.02.001

• 论著 •    下一篇

硫化氢对创伤应激引起大鼠急性肝损伤的作用

岑新海1，2，张志湘2，王  涛1，王艳莎1，季英磊1，2，闫  骏1，2，谷振勇1，2   

  1. （1. 南通大学医学院法医学系，江苏 南通 226001； 2. 苏州大学医学部，江苏 苏州 215123）
  • 发布日期:2016-04-25 出版日期:2016-04-28
  • 通讯作者: 谷振勇，男，博士，教授，博士研究生导师，主要从事T-MODS、血管生物学和呼吸病理生理分子机制的研究；E-mail：zygusz@126.com
  • 作者简介:岑新海（1975—），男，博士研究生，主要从事T-MODS机制研究
  • 基金资助:

    国家自然科学基金资助项目（81273341）；江苏省高校优势学科建设工程项目（PAPD）

The Role of Hydrogen Sulfide in Acute Liver Injury Induced by Traumatic Stress in Rats

CEN XIN-HAI1,2, ZHANG ZHI-XIANG2, WANG TAO1, WANG YAN-SHA1, JI YING-LEI1,2, YAN JUN1,2, GU ZHEN-YONG1,2   

  1. （1. Department of Forensic Medicine, Medical College of Nantong University, Nantong 226001, China; 2. Medical College of Soochow University, Suzhou 215123, China）
  • Online:2016-04-25 Published:2016-04-28

被引次数

7

摘要： 目的 探讨硫化氢（H2S）在挤压大鼠后肢致急性肝损伤过程中的作用。 方法 将大鼠随机分为对照组、挤压组、H2S供体硫氢化钠（NaHS）+挤压组、H2S生成抑制剂炔丙基甘氨酸（propargylglycine，PAG）+挤压组。用标准重物挤压大鼠后肢建立急性肝损伤模型，分别于挤压结束后30、120 min处死大鼠。比色法检测血清天门冬氨酸氨基转移酶（AST）、丙氨酸氨基转移酶（ALT）活性，化学法检测大鼠血浆H2S、肝组织丙二醛（malondialdehyde，MDA）、蛋白质羰基、谷胱甘肽（glutathion，GSH）含量和H2S生成酶胱硫醚γ-裂解酶（cystathionine γ-lyase，CSE）活性，RT-PCR半定量检测肝组织CSE mRNA的表达。 结果 挤压大鼠后肢可引起血清AST、ALT活性增高，肝组织MDA、蛋白质羰基含量增加及GSH含量降低，血浆H2S含量减少，肝组织CSE活性降低、CSE mRNA表达下降。预先给予NaHS可显著减轻、而PAG明显加剧挤压后肢所致上述肝损伤改变。 结论 H2S生成减少参与介导创伤应激引起的大鼠急性肝损伤。

关键词: 法医病理学, 创伤和损伤, 肝, 应激, 硫化氢, 大鼠

Abstract: Objective To explore the role of hydrogen sulfide （H2S） in acute liver injury induced by crushing hind limbs of rats. Methods The rats were randomly divided into the following groups: control, crushing, H2S donor sodium hydrosulfide （NaHS） + crushing, H2S inhibitor propargylglycine （PAG） + crushing group. The acute liver injury model was established by crushing the hind limbs of rats with standard weight. Rats were sacrificed at 30 min and 120 min after the crush. The activities of serum aspartate aminotransferase （AST） and alanine aminotransferase （ALT） were measured by colorimetric method, and the content of H2S in plasma and the contents of malondialdehyde （MDA）, protein carbonyl, glutathione （GSH） in the liver and the activity of H2S generating enzyme （cystathionine γ-lyase, CSE） were determined by chemical method. The expression of CSE mRNA in liver was detected by RT-PCR. Results For crush injury group, the levels of AST and ALT in serum, MDA and protein carbonyl in liver increased. The levels of GSH, CSE, CSE mRNA in liver and H2S in serum decreased. The administration of NaHS before limbs crush could attenuate the changes of liver injury, but the pre-treatment with PAG could exacerbate the changes. Conclusion The decrease of H2S production could involve in mediating the acute liver injury induced by traumatic stress in rats.

Key words: forensic pathology, wounds and injuries, liver, stress, hydrogen sulfide, rats

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