法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (6): 415-419.DOI: 10.3969/j.issn.1004-5619.2016.06.005

• 论著 • 上一篇    下一篇

XPG基因表达用于法医学年龄推断

邓小冬1，张  伟1，张  波1，马  英2，木尔扯尔1，章丽霞1，谢  英1，刘  云1   

  1. （1. 川北医学院法医学系，四川 南充 637000； 2. 川北医学院附属医院神经内科，四川 南充 637000）
  • 发布日期:2016-12-25 出版日期:2016-12-28
  • 通讯作者: 刘云，男，博士，教授，主要从事法医病理学和法医毒理学研究；E-mail：xyun2005@163.com
  • 作者简介:邓小冬（1988—），男，硕士，助教，主要从事法医学研究；E-mail：857123409@qq.com
  • 基金资助:

    国家自然科学基金青年科学基金资助项目（81400966）；四川省教育厅科研基金重点项目（14ZA0191，15ZA0209）

Expression of XPG Gene in Forensic Age Estimation

DENG XIAO-DONG1, ZHANG WEI1, ZHANG BO1, MA YING2, MUER CHE-ER1, ZHANG LI-XIA1, XIE YING1, LIU YUN1   

  1. （1. Department of Forensic Medicine, North Sichuan Medical College, Nanchong 637000, China; 2. Department of Neurology, Affiliated Hospital of North Sichuan Medical College, Nanchong 637000, China）
  • Online:2016-12-25 Published:2016-12-28

摘要： 目的 探讨着色性干皮病G组（xeroderma pigmentosum group G，XPG）基因在不同年龄段健康汉族人群中的表达情况，分析XPG mRNA和蛋白表达量与年龄之间的相关性，以期为法医学年龄推断提供新的分子生物学指标。 方法 收集150名不同年龄段健康汉族人的外周血样，采用TRIzol法提取外周血单个核细胞（peripheral blood mononuclear cell，PBMC）总RNA，通过实时荧光定量PCR检测XPG mRNA在PBMC的相对表达量，酶联免疫吸附试验检测XPG蛋白在血浆中的表达量。 结果 XPG mRNA及其蛋白表达量在≤18岁组与19~45岁组之间、≤18岁组与≥46岁组之间的差异均有统计学意义（P<0.05），但19~45岁组与≥46岁组之间的差异无统计学意义（P>0.05）。XPG mRNA和蛋白表达量均无性别差异（P>0.05）。 结论 XPG mRNA在PBMC的相对表达量在低龄段内随年龄增加而下降，其血浆中蛋白随年龄增加而升高；XPG基因有望成为法医学年龄推断的新型指标之一。

关键词: 法医遗传学, 法医人类学, 着色性干皮病G组, 实时荧光定量PCR, 酶联免疫吸附试验, 年龄推断

Abstract: Objective To explore the expression of xeroderma pigmentosum complementation group G （XPG） gene in healthy Han population of different ages and to analysis the relationship between the mRNA and protein expression levels of XPG and age, which may provide a new molecular-biological indicator for forensic age determination. Methods Total 150 samples of peripheral blood were collected from healthy Han population of different ages. Total RNA of peripheral blood mononuclear cell （PBMC） were extracted by TRIzol method, and the relative expression of XPG mRNA in PBMC was detected by quantitative real-time PCR, and the protein expression levels of XPG in plasma were detected by ELISA. Results The mRNA and protein expression levels of XPG in ≤18 years old group were significantly different from 19-45 years old group and ≥46 years old group （P<0.05）, while there was no significant difference between 19-45 years old group and ≥46 years old group （P>0.05）. No significant sex differences were observed in mRNA and protein expression levels of XPG （P>0.05）. Conclusion The relative expression level of XPG mRNA in PBMC declines with the increase of age in younger age, while the protein expression level in plasma increases with age, and XPG gene can be used as one of new markers for forensic age estimation.

Key words: forensic genetics, forensic anthropology, xeroderma pigmentosum group G, quantitative real-time PCR, enzyme linked immunosorbent assay, age estimation

中图分类号: