法医学杂志

法医学杂志 ›› 2016, Vol. 32 ›› Issue (6): 420-423.DOI: 10.3969/j.issn.1004-5619.2016.06.006

• 论著 • 上一篇    下一篇

16个X-STR基因座在河南汉族人群中的法医学应用评估

刘亚举1，郭利红2，岳俊涛1，石美森3   

  1. （1. 许昌市公安局刑事科学技术研究所，河南 许昌 461000； 2. 河南省公安厅刑事科学技术研究所，河南 郑州 450003； 3. 中国政法大学 证据科学教育部重点实验室，北京 100088）
  • 发布日期:2016-12-25 出版日期:2016-12-28
  • 通讯作者: 石美森，女，博士，教授，硕士研究生导师，主要从事法医物证学和群体遗传学研究；E-mail：shimeisen2000@163.com
  • 作者简介:刘亚举（1978—），男，副主任法医师，主要从事法医DNA检验鉴定和群体遗传学研究；E-mail：horse3697@126.com

Forensic Application of 16 X-STR Loci in Henan Han Population

LIU YA-JU1, GUO LI-HONG2, YUE JUN-TAO1, SHI MEI-SEN3   

  1. （1. Institute of Criminal Science and Technology, Xuchang Public Security Bureau, Xuchang 461000, China; 2. Institute of Criminal Science and Technology, Henan Public Security Bureau, Zhengzhou 450003, China; 3. Key Laboratory of Evidence Science, Ministry of Education, China University of Political Science and Law, Beijing 100088, China）
  • Online:2016-12-25 Published:2016-12-28

被引次数

2

摘要： 目的 调查16个X-STR基因座在河南汉族人群中的遗传学数据，评估其法医学应用价值。 方法 应用GoldeneyeTM DNA身份鉴定系统17X试剂盒，对河南地区326名汉族无关个体DNA进行PCR扩增，3130xl型遗传分析仪电泳分析，GeneMapper?誖ID-X软件分析等位基因片段大小。统计分析16个X-STR基因座的频率数据和群体遗传学参数，并与其他地区已有人群数据进行比较。 结果 在所检测的16个X-STR基因座中，DXS6800具有中度多态性，其余15个基因座均具有高度多态性。这16个X-STR基因座在女性群体的累积个人识别率为0.999 999 999 999 992，在男性群体的累积个人识别率为0.999 999 996 577 712，在三联体中的累积非父排除率为0.999 999 971，在二联体中的累积非父排除率为0.999 992 574。 结论 这16个X-STR基因座达到了法医物证学应用要求，尤其对特殊的亲权鉴定案件具有重要的应用价值。

关键词: 法医遗传学, 多态现象, 遗传, X-STR, 河南, 汉族

Abstract: Objective To investigate the genetic data of 16 X-STR loci in Henan Han population and to assess the application value in forensic science. Methods The DNA of 326 unrelated individuals in Henan Han population were amplified using GoldeneyeTM DNA identification system 17X kit, and the PCR products were analyzed by electrophoresis through 3130xl genetic analyzer. The fragment sizes of alleles were analyzed subsequently by GeneMapper?誖ID-X. Allele frequencies and population genetics parameters of 16 X-STR loci were analyzed statistically and compared with the available data of other Han populations from different regions. Results Among the 16 X-STR loci, DXS6800 were found to be moderately polymorphic and the other 15 X-STR loci were highly polymorphic. The cumulative discrimination power in females and males were 0.999 999 999 999 992 and 0.999 999 996 577 712, respectively. The combined power of exclusion in trios and in duos were 0.999 999 971 and 0.999 992 574, respectively. Conclusion The 16 X-STR loci meet the application requires of forensic genetics, especially for testing the special paternity cases.

Key words:  forensic genetics, polymorphism, genetic, X-STR, Henan, Han nationality

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