法医学杂志

法医学杂志 ›› 2017, Vol. 33 ›› Issue (3): 281-282.DOI: 10.3969/j.issn.1004-5619.2017.03.014

• 技术与应用 • 上一篇    下一篇

直接扩增法用于重大灾难事故中软骨的个体识别

王传海，徐  程，李湘秦，吴  勇，杜  舟   

  1. （深圳市公安局刑事警察支队刑事技术处，广东 深圳 518040）
  • 发布日期:2017-06-25 出版日期:2017-06-28
  • 通讯作者: 徐程，女，硕士，主要从事法医DNA检验和鉴定；E-mail：xucheng19891020@aliyun.com
  • 作者简介:王传海（1973—），男，硕士，副主任法医师，主要从事法医DNA检验和鉴定；E-mail：boatseawang@126.com

Individual Identification of Cartilage by Direct Amplification in Mass Disasters

WANG CHUAN-HAI, XU CHENG, LI XIANG-QIN, WU YONG, DU ZHOU   

  1. （Criminal Technology Division, Criminal Police Detachment, Shenzhen Public Security Bureau, Shenzhen 518040, China）
  • Online:2017-06-25 Published:2017-06-28

摘要： 目的 探讨直接扩增法对软骨STR检验的有效性，以提高灾难受害者身源鉴定工作效率。 方法 采用PowerPlex?誖21试剂盒对88份软骨进行直接扩增法检验，同步进行磁珠法比较分型结果。 结果 88份软骨检材中，直接扩增法与磁珠法均成功检出84份检材的STR基因型，两者分型结果完全一致。 结论 PowerPlex?誖21试剂盒直接扩增法可以应用于软骨STR分型检验，且操作简便、快速，在重大灾难事故身源鉴定中具有很好的应用前景。

关键词: 法医遗传学, 软骨, 基因分型技术, 直接扩增法, 个体识别

Abstract: Objective To explore the effectiveness of direct amplification for the STR analysis of cartilage, and to accelerate the effectiveness of disaster victim identification. Methods Eighty-eight cartilage samples were directly amplified by PowerPlex?誖21 kit, and the results of genotyping were compared with that obtained by the magnetic beads method. Results In 88 cartilage samples, the STR genotypes were successfully detected from 84 samples by direct amplification and magnetic beads method, and both the results of genotyping by two method were consistent. Conclusion Direct amplification with PowerPlex?誖21 kit can be used for STR genotyping of cartilages. This method is operated easily and promptly, which has a potential application in the individual identification of mass disasters.

Key words: forensic genetics, cartilage, genotyping techniques, direct amplification, individual identification

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