法医学杂志

法医学杂志 ›› 2017, Vol. 33 ›› Issue (4): 357-362.DOI: 10.3969/j.issn.1004-5619.2017.04.005

• 论著 • 上一篇    下一篇

淮海战役士兵遗骸的Y染色体遗传类型鉴定

王迟早1，文少卿1，石美森2，俞雪儿1，万雪娇3，潘伊凌3，张云飞3，李  辉1，谭婧泽1   

  1. （1. 复旦大学生命科学学院现代人类学教育部重点实验室，上海 200438； 2. 中国政法大学证据科学教育部重点实验室，北京 100088； 3. 上海科技馆自然史研究中心，上海 200127）
  • 发布日期:2017-08-25 出版日期:2017-08-28
  • 通讯作者: 谭婧泽，女，副教授，主要从事体质人类学研究；E-mail：jztan@fudan.edu.cn
  • 作者简介:王迟早（1990—），女，硕士研究生，主要从事分子人类学研究；E-mail：wangchizao@126.com 通信作者：李辉，男，教授，主要从事分子人类学研究；E-mail：LHCA@fudan.edu.cn
  • 基金资助:

    国家自然科学基金资助项目（31222030，31371266）；教育部科学技术研究资助项目（113022A）；曙光计划资助项目（14SG05）；复旦大学遗传工程国家重点实验室开放课题资助项目（SKLGE-1401）；中国科学院计算生物学重点实验室开放课题资助项目

Identification of Y-chromosomal Genetic Types for the Soldier’s Remains from Huaihai Campaign

WANG CHI-ZAO1, WEN SHAO-QING1, SHI MEI-SEN2, YU XUE-ER1, WAN XUE-JIAO3, PAN YI-LING3, ZHANG YUN-FEI3, LI HUI1, TAN JING-ZE1   

  1. （1. MOE Key Laboratory of Contemporary Anthropology, School of Life Sciences, Fudan University, Shanghai 200438, China; 2. Key Laboratory of Evidence Law and Forensic Science, Ministry of Education, China University of Political Science and Law, Beijing 100088, China; 3. Natural History Research Center, Shanghai Science and Technology Museum, Shanghai 200127, China）
  • Online:2017-08-25 Published:2017-08-28

摘要： 目的 鉴定淮海战役士兵遗骸的Y染色体遗传类型，为寻找其父系亲属提供线索。 方法 采用古DNA的方法提取遗骸DNA，使用Yfiler试剂盒进行17个Y-STR基因座的复合扩增，推测样本的单倍群，并根据最新Y染色体谱系树挑选Y-SNP位点进行精细分型，再基于Y-SNP和Y-STR数据进行共享单倍型分析，获得与遗骸遗传关系最近的现代个体信息。 结果 8份男性样本中的17个Y-STR基因座总共观察到8种Y-STR单倍型，进一步Y-SNP分析得出6种Y-SNP单倍群，分别是O2a1-M95+、O1a1-P203+、O3*-M122+/M234-、D1-M15+、C3*-ST和R1a1-M17+。 结论 本次对淮海战役士兵遗骸进行的Y染色体遗传类型鉴定对于推断陈年检材的地理来源具有一定的借鉴价值。

关键词:  , 法医遗传学, Y染色体, 军事人员, 个体识别, 淮海战役

Abstract: Objective To identify the Y-chromosomal genetic types for the soldier’s remains from Huaihai Campaign, and to offer a clue for search of their paternal relatives. Methods DNA of the remains were extracted by the ancient DNA extraction method. Yfiler kit was used for the multiplex amplification of 17 Y-STR loci. The haplogroups of the samples were speculated. Detailed genotyping of the selected Y-SNP was performed based on the latest Y-chromosome phylogenetic tree. Haplotype-sharing analysis was done based on the data of Y-SNP and Y-STR, the closest modern individual information to the genetic relationship of remains was gained. Results A total of 8 Y-STR haplotypes were observed on 17 Y-STR loci of 8 male individuals. Furthermore, 6 Y-SNP haplogroups were identified, which were O2a1-M95+, O1a1-P203+, O3*-M122+/M234-, D1-M15+, C3*-ST and R1a1-M17+. Conclusion Identification of Y-chromosomal genetic types for the soldier’s remains from Huaihai Campaign shows a reference value on inferring the geographical origins of old materials.

Key words: forensic genetics, Y chromosome, military personnel, personal identification, Huaihai Campaign

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