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法医学杂志 2017, Vol. 33 Issue (06) :607-610    DOI: 10.3969/j.issn.1004-5619.2017.06.008
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D18S51及其侧翼区3个SNP组成的SNP-STR在亲子鉴定中的应用
高泾尚1,叶懿2,侯一平2
(1. 西藏自治区公安厅物证鉴定中心,西藏 拉萨 850000; 2. 四川大学华西基础医学与法医学院,四川 成都 610041)
Application of SNP-STR Composed by D18S51 and Three SNPs of Its Flanking Region in Paternity Testing
GAO Jing-shang1, YE Yi2, HOU Yi-ping2
(1. Institute of Forensic Science, Tibet Autonomous Region Public Security Department, Lhasa 850000, China; 2. West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China)

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摘要 目的 将一个单倍型区块内的遗传标记单核苷酸多态性(SNP)和短串联重复序列(STR)组成SNP-STR单倍型,调查其在成都汉族人群中的分布,并探讨其在特殊亲子鉴定案例中的应用价值。 方法 选取DNA联合索引系统(combined DNA index system,CODIS)中突变率较高的基因座D18S51,与其侧翼区的3个SNP位点(rs8089331、rs8094489、rs7236090)组成SNP-STR,通过巢式等位基因特异性PCR的方法获得SNP-STR单倍型,调查该单倍型在75名成都汉族人群中的分布,并应用于两例D18S51基因座不符合遗传规律的二联体亲子鉴定案件。 结果 成功建立SNP-STR分型方法,在成都汉族人群中共发现43种单倍型,多态性为0.948 6,并成功解决了两例二联体亲子鉴定案件。 结论 SNP-STR具有良好的多态性,有望应用于特殊的亲缘关系鉴定。
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高泾尚
叶懿
侯一平
关键词法医遗传学   短串联重复序列   单核苷酸多态性   遗传标记   组合   成都   汉族     
Abstract: Objective To develop a SNP-STR haplotype by consisting of the SNP and STR genetic markers, both of which locate in a haplotype block. To investigate its distribution in Han population from Chengdu, and explore its application in some special cases of paternity testing. Methods D18S51, one of the high mutation rate STR markers in combined DNA index system (CODIS), and three SNP loci rs8089331, rs8094489 and rs7236090 in its flanking region, were chosen to establish SNP-STR. Its haplotype was obtained by nested allele-specific polymerase chain reaction, and the relevant distribution of 75 unrelated individuals were investigated in Han population from Chengdu. The SNP-STR haplotype was tentatively applied in duo paternity testing cases with D18S51 incompatibility. Results The SNP-STR typing method was established and a total of 43 haplotypes were obtained successfully in Han population from Chengdu. Its polymorphism was 0.948 6, and duo paternity testing cases were resolved by this method. Conclusion SNP-STR shows high diversity and can be applied in the identifications of some special paternity testing cases.
Keywordsforensic genetics   short tandem repeat   single nucleotide polymorphism   genetic markers   combination   Chengdu   Han population     
Fund:

国家自然科学基金资助项目(81401557,81330073)

Corresponding Authors: 侯一平,男,教授,博士研究生导师,主要从事法医遗传学研究;E-mail:forensic@scu.edu.cn   
About author: 高泾尚(1979—),男,主检法医师,主要从事法医病理、损伤和法医物证学研究;E-mail:35323811@qq.com
引用本文:   
高泾尚, 叶懿, 侯一平.D18S51及其侧翼区3个SNP组成的SNP-STR在亲子鉴定中的应用[J]  法医学杂志, 2017,V33(06): 607-610
GAO Jing-Shang, YE Yi, HOU Yi-Ping.Application of SNP-STR Composed by D18S51 and Three SNPs of Its Flanking Region in Paternity Testing[J]  , 2017,V33(06): 607-610
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