法医学杂志

法医学杂志 ›› 2018, Vol. 34 ›› Issue (5): 532-537.DOI: 10.12116/j.issn.1004-5619.2018.05.019

• 技术与应用 • 上一篇    下一篇

miniSTR基因座及其检测系统在降解检材中的法医学应用

王  鑫1，陈维忠1，张  健1，李景辉1，孙元鹏1，石云杰1，张  雷2，陈林丽2，周  翔2，周如华1   

  1. 1． 苏州市公安局物证鉴定所，江苏 苏州 215131； 2． 无锡中德美联生物技术有限公司，江苏 无锡 214174
  • 发布日期:2018-10-25 出版日期:2018-10-28
  • 通讯作者: 周如华，男，副主任法医师，主要从事法医物证学检验；E-mail：13961329595@139.com
  • 作者简介:王鑫（1986—），男，主检法医师，主要从事法医物证学检验；E-mail：wangxin5552137@163.com
  • 基金资助:
    苏州市科学技术局产业技术创新专项（民生科技）资助项目（SS201526）

Application of miniSTR Loci and Its Detection System for Degraded Materials in Forensic Medicine#br#

WANG Xin1, CHEN Wei-zhong1, ZHANG Jian1, LI Jing-hui1, SUN Yuan-peng1, SHI Yun-jie1, ZHANG Lei2, CHEN Lin-li2, ZHOU Xiang2, ZHOU Ru-hua1   

  1. 1. Institute of Forensic Science, Suzhou Public Security Bureau, Suzhou 215131, Jiangsu Province, China; 2. AGCU ScienTech Incorporation, Wuxi 214174, Jiangsu Province, China
  • Online:2018-10-25 Published:2018-10-28

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摘要： 目的 建立16个miniSTR基因座的复合扩增体系，探讨其在法医学降解检材中的应用价值。 方法 采用六色荧光标记技术，对16个miniSTR基因座进行复合扩增体系的构建及法医学应用评估。 结果 开发出一个六色荧光标记的miniSTR扩增试剂盒，可同时对15个常染色体STR基因座、Amelogenin基因座和DYS391基因座进行分型检测，方法特异性好，分型结果稳定、准确，灵敏度达50 pg，实际案件中常见生物检材的检验结果良好。 结论 16个miniSTR复合扩增体系对降解与微量检材的检测具有应用价值，灵敏度高，具有数据库兼容性，可以用于实际案件检验。

关键词: 法医遗传学, 短串联重复, 降解检材, 微量检材, 复合扩增体系

Abstract: Objective To establish multiplex system of 16 miniSTR loci, and explore its application value for the degraded materials in forensic medicine. Methods The multiplex system of 16 miniSTR loci was established using a six-dye fluorescence labeling technology and its application value in forensic medicine was assessed. Results A six-dye fluorescence labeling miniSTR amplification kit was developed, which enabled 15 autosomal STR loci, Amelogenin locus and DYS391 to be typed simultaneously. This method showed good specificity and could provide stable and accurate typing results with a sensitivity of 50 pg. This system also provided a good test result for the normal biological sample of actual cases. Conclusion The multiplex system of 16 miniSTR loci has application value for degraded and trace materials with the advantages of high sensitivity and database compatibility, which can be used for forensic casework.

Key words: forensic genetics, short tandem repeat, degraded DNA, low-level DNA, multiplex kits