法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (2): 224-229.DOI: 10.12116/j.issn.1004-5619.2019.02.017

• 论著 • 上一篇    下一篇

及己根、茎、叶醇提取物的心脏毒性和毒性机制

孙淑萍1，2，3， 李红星1，3， 张小平2， 马云飞1， 杨梅1，3   

  1. 1. 皖南医学院药学院，安徽 芜湖 241002； 2. 安徽师范大学生命科学院，安徽 芜湖 241000； 3. 皖南医学院天然日化研究所，安徽 芜湖 241002
  • 发布日期:2019-04-25 出版日期:2019-04-28
  • 通讯作者: 张小平，男，博士，教授，博士研究生导师，主要从事植物分类与系统进化、植物保护生物学和进化生态学方面的研究；E-mail：pinghengxu@sina.com.cn
  • 作者简介:孙淑萍（1974—），女，博士研究生，执业药师，副教授，硕士研究生导师，主要从事中药药效和毒理的研究；E-mail：sun5587001@163.com
  • 基金资助:
    国家自然科学基金资助项目（91742101）；安徽省自然科学基金资助项目（1608085MH160）；安徽省卫生计生委中医药课题资助项目（2016zy95）；安徽省科技厅重点研究与开发资助项目（1804h08020271）；皖南医学院学科带头人后备人选资助项目（皖南医学院校政2015年20号）

Mechanisms of Toxicity and Cardiotoxicity of Alcohol Extract from Root, Stem and Leaf of Chloranthus Serratus

SUN Shu-ping1,2,3, LI Hong-xing1,3, ZHANG Xiao-ping2, MA Yun-fei1, YANG Mei1,3   

  1. 1. College of Pharmacy, Wannan Medical College, Wuhu 241002, Anhui Province, China; 2. College of Life Sciences, Anhui Normal University, Wuhu 241000, Anhui Province, China; 3. Institute of Natural Daily Chemistry, Wannan Medical College, Wuhu 241000, Anhui Province, China
  • Online:2019-04-25 Published:2019-04-28

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摘要： 目的 比较及己根、茎、叶醇提取物对大鼠心脏毒性的差异，初步探讨其毒性机制。 方法 将大鼠随机分成空白组、根醇组、茎醇组和叶醇组，每组8只。连续灌胃给药14 d后，绘制体质量变化曲线，计算心脏系数，检测各组大鼠血清中肌酸激酶（creatine kinase，CK）、肌酸激酶同工酶（CK-MB）、乳酸脱氢酶（lactate dehydrogenase，LDH）和α-羟丁酸脱氢酶（α-hydroxybutyrate dehydrogenase，α-HBDH）的含量及心脏组织中氧化应激指标［总超氧化物歧化酶（total superoxide dismutase，T-SOD）和丙二醛（malondialdehyde，MDA）］的含量变化，观察各组大鼠心脏的组织病理学改变，检测心肌组织中细胞间黏附分子-1（intercellular adhesion molecule-1，ICAM-1）、血红素加氧酶-1（heme oxygenase-1，HO-1）的表达。 结果 体质量增长率为茎醇组最小，其次为叶醇组。心脏系数在各组间差异均无统计学意义（P>0.05）。茎醇组心肌组织损伤程度最严重，其次为叶醇组。茎醇组CK、CK-MB、LDH、α-HBDH含量均升高（P<0.05）。与空白组和根醇组相比，茎醇组MDA含量升高，T-SOD含量降低，叶醇组仅T-SOD含量较空白组降低（P<0.05）。不同提取物干预后，ICAM-1阳性表达均增强，HO-1蛋白表达均降低，变化趋势为茎醇组>叶醇组>根醇组。 结论 及己茎醇提取物的心脏毒性最大，其次为叶醇提取物，其毒性机制可能与氧化应激有关。

关键词: 法医病理学, 法医毒理学, 及己, 植物提取物, 毒性作用, 大鼠

Abstract:

Objective To compare the differences of cardiotoxicity of alcohol extract from root, stem and leaf of Chloranthus serratus in the rats, and discuss preliminarily its mechanism of toxicity. Methods Rats were randomly divided into four groups: blank, root alcohol, stem alcohol and leaf alcohol, with 8 in each group. After 14 days of continuous intragastric administration, the body mass change curves were drawn. The cardiac coefficient was calculated. The contents of creatine kinase （CK）, creatine kinase isoenzyme （CK-MB）, lactate dehydrogenase （LDH） and α-hydroxybutyrate dehydrogenase （α-HBDH） as well as the content changes of oxidative stress indexes — total superoxide dismutase （T-SOD） and malondialdehyde （MDA） in the serum of rats were detected. The cardiac pathomorphology changes in the rats were observed. The expression of intercellular adhesion molecule （ICAM-1） and heme oxygenase （HO-1） in myocardial tissue was detected. Results Body mass growth rate： stem alcohol group was the smallest, followed by leaf alcohol group. The difference of cardiac coefficient of every group had no statistical significance （P>0.05）. The myocardial tissues of stem alcohol group suffered the most serious damage, followed by the leaf alcohol group. The contents of CK, CK-MB, LDH and α-HBDH in stem alcohol group increased （P<0.05）. The increase of MDA content and decrease of T-SOD content in stem alcohol group had statistical significance compared with the blank group and root alcohol group, while the leaf alcohol group only had statistical significance in the decrease of T-SOD content compared with the blank group （P<0.05）. The positive expression of ICAM-1 enhanced and the expression of HO-1 protein decreased in every group after the intervention of different extracts. The change trend was stem alcohol > leaf alcohol > root alcohol group. Conclusion The alcohol extract from the stem has the highest cardiotoxicity, followed by the leaf extract, and its mechanism of toxicity may be related to oxidative stress.

Key words: forensic pathology, forensic toxicology, Chloranthus serratus, plant extracts, toxic actions, rats