法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (3): 314-318.DOI: 10.12116/j.issn.1004-5619.2019.03.010

• 技术与应用 • 上一篇    下一篇

广西6个民族Y-STR网络图分析及其法医学意义

肖月1， 邓盼2， 常开创3， 马泉4， 钱恩芳4，5， 余建华6， 程宝文7， 李彩霞4， 江丽4   

  1. 1. 广东中一司法鉴定中心，广东 深圳 518000； 2. 株洲市公安局，湖南 株洲 412007； 3. 昆明市公安局，云南 昆明 650034； 4. 公安部物证鉴定中心 北京市现场物证检验工程技术研究中心 现场物证溯源技术国家工程实验室，北京 100038； 5. 贵州医科大学法医学院，贵州 贵阳 550004； 6. 昭通市公安局，云南 昭通 657000； 7. 云南警官学院，云南 昆明 650221
  • 发布日期:2019-06-25 出版日期:2019-06-28
  • 通讯作者: 李彩霞，女，博士，主任法医师，主要从事法医物证学研究及鉴定；E-mail：licaixia@tsinghua.org.cn 江丽，女，博士，副主任法医师，主要从事法医物证学研究及鉴定；E-mail：jl@mail.bnu.edu.cn
  • 作者简介:肖月（1992—），女，硕士研究生，主要从事法医物证学鉴定和研究；E-mail：625287645@qq.com
  • 基金资助:
    国家重点研发计划资助项目（2017YFC0803501）；国家自然科学基金资助项目（81772027）；公安部技术研究计划资助项目（2015JSYJC47，NO2016JSYJA05）；国家科技资源共享服务平台计划资助项目（YCZYPT[2017]01-3）；公安部物证鉴定中心基本科研业务费专项资助项目（2017JB027，2016JB039，2017JB025）

Network Analysis of Y-STR in Six Ethnic Populations in Guangxi and Its Forensic Significances

XIAO Yue1, DENG Pan2, CHANG Kai-chuang3, MA Quan4, QIAN En-fang4,5, YU Jian-hua6, CHENG Bao-wen7, LI Cai-xia4, JIANG Li4   

  1. 1. Guangdong Zhongyi Forensic Science Center, Shenzhen 518000, Guangdong Province, China; 2. Zhuzhou Municipal Public Security Bureau, Zhuzhou 412007, Hunan Province, China; 3. Kunming Municipal Public Security Bureau, Kunming 650034, China; 4. Key Laboratory of Forensic Genetics, National Engineering Laboratory for Forensic Science, Beijing Engineering Research Center of Crime Scene Evidence Examination, Institute of Forensic Science, Ministry of Public Security, PRC, Beijing 100038, China; 5. Institute of Forensic Medicine, Guizhou Medical University, Guiyang 550004, China; 6. Zhaotong Municipal Public Security Bureau, Zhaotong 657000, Yunnan Province, China; 7. Yunnan Police Officer Academy, Kunming 650221, China
  • Online:2019-06-25 Published:2019-06-28

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摘要： 目的 探究Y-SNP和Y-STR遗传标记信息在不同民族中的遗传结构分布及其法医学应用。 方法 采用SNaPshot微测序技术对广西汉族、广西京族、广西苗族、广西瑶族、广西壮族和广西侗族6个民族439名男性个体12个Y-SNP位点多态性进行检测，采用DNATyperTM Y26试剂盒对26个Y-STR基因座进行复合扩增，3130xl基因分析仪进行基因分型。对同一Y-SNP单倍群下不同个体Y-STR单倍型使用Network 5.0绘制网络图并分析。 结果 12个Y-SNP位点在6个民族中检出6个单倍群，26个Y-STR基因座检出362个单倍型，单倍型多样性为0.996 6。C单倍群中，广西瑶族、广西壮族和广西侗族样本聚集在不同簇上；O1单倍群中，广西壮族、广西苗族和广西京族样本相对独立并分别聚集一簇；O2单倍群中部分广西苗族和广西瑶族样本聚集一簇。 结论 基于同一Y-SNP单倍群下Y-STR网络图分析，能初步对部分民族进行区分，为相关地区利用犯罪现场遗留男性生物检材的检验推断族群来源。

关键词: 法医遗传学, 多态性, 单核苷酸, Y染色体, 短串联重复, 单倍型, 广西

Abstract: Objective To explore the distribution of genetic structure of Y-SNP and Y-STR genetic markers in different ethnic groups and its application in forensic science. Methods SNaPshot minisequencing was used to detect the polymorphisms of 12 Y-SNP loci in 439 males from 6 ethnic groups, including Guangxi Han, Guangxi Jing, Guangxi Miao, Guangxi Yao, Guangxi Zhuang and Guangxi Dong. DNATyperTM Y26 kit was used to multiplex-amplify 26 Y-STR loci. The PCR products were analyzed by 3130xl genetic analyzer. The network analysis of Y-STR haplotype under the same Y-SNP haplogroup was analyzed by Network 5.0 software. Results Six haplogroups defined by 12 Y-SNP loci were detected in 6 ethnic groups, and 362 haplotypes were detected in 26 Y-STR loci. The haplotype diversity was 0.996 6. In the C haplogroup, the samples from Guangxi Yao, Guangxi Zhuang and Guangxi Dong were clustered on different branches; in the O1 haplogroup, those from Guangxi Zhuang, Guangxi Miao and Guangxi Jing were relatively independent and clustered separately; in the O2 haplogroup, some samples from Guangxi Miao and Guangxi Yao were gathered in a cluster. Conclusion Based on the Y-STR network analysis of samples with identical haplogroup of Y-SNP, some ethnic groups can be preliminarily distinguished, which could be used to infer male suspects’ ethnic group through detecting their genetic markers left in the crime scene.

Key words: forensic genetics, polymorphism, single nucleotide, Y chromosome, short tandem repeat, haplotype, Guangxi