Postmortem Distribution and Postmortem Redistribution of Carbofuran-7-Phenyl Glucuronic Acid in Rabbits

doi:10.12116/j.issn.1004-5619.2020.301204

Abstract

Abstract:

Objective To establish a carbofuran intragastric administration death model in rabbits, and to observe the postmortem distribution and postmortem redistribution of carbofuran-7-phenyl glucuronic acid （Glu-7PH） in rabbits. Methods The postmortem distribution: Rabbits were given an administration of 1/2LD50, LD50, 2LD50 carbofuran. Dead rabbits were dissected immediately. Rabbits that had remained alive 2 hours were sacrificed by carbon dioxide （CO₂） inhalation and dissected immediately. The myocardium, cardiac blood, liver, spleen, lung, kidney, brain and right hindlimb muscle were collected. The postmortem redistribution: After giving an administration of 4LD50 carbofuran, the myocardium, cardiac blood, liver, spleen, lung, kidney, brain, and right hindlimb muscle were collected at 0, 12, 24, 48, and 72 h postmortem in supine position at 15 ℃ room temperature. The quantity of Glu-7PH was determined by LC-MS/MS. Results The postmortem distribution: Among the three dose groups, there were significant differences in the quantities of Glu-7PH in different tissues. The postmortem redistribution: There was no significant difference in the Glu-7PH quantities in cardiac blood, mycardium, spleen, kidney, brain and right hindlimb muscle, but there was a significant difference in the Glu-7PH quantities in the liver and lung. Conclusion The mycardium, cardiac blood, liver, lung, kidney, brain and hindlimb muscle of rabbits can be used as appropriate samples for Glu-7PH detection. However, it should be noted that Glu-7PH was redistributed postmortem in rabbit liver and lung.

Key words: forensic toxicological, carbofuran-7-phenyl glucuronic acid, postmortem distribution, postmortem redistribution, liquid chromatography-tandem mass spectrometry（LC-MS/MS）, rabbits

CLC Number:

DF795.1

Yan WEN, Yu-zhe HAN, Duo GONG, Wen-kai XIE, Chen-xi LÜ, Yu-zhen MENG, Chao ZHANG, Zhi-wen WEI, Ke-ming YUN. Postmortem Distribution and Postmortem Redistribution of Carbofuran-7-Phenyl Glucuronic Acid in Rabbits[J]. Journal of Forensic Medicine, 2022, 38(5): 601-605.

Figures/Tables 2

References 22

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组织	1/2LD50	LD50	2LD50
心血/（ng·mL^-1）	35.51±14.51^1）	70.40±44.34^1）	205.31±79.88
心肌/（ng·mg^-1）	37.22±12.69^1）	102.51±80.01	281.02±125.94
肝/（ng·mg^-1）	84.76±63.17	191.07±147.95	821.80±527.51^2）
脾/（ng·mg^-1）	-	45.68±29.92^1）	191.81±115.76
肺/（ng·mg^-1）	37.98±15.95^1）	107.97±113.80	250.19±109.38
肾/（ng·mg^-1）	395.66±167.14	798.11±506.41	2 646.55±1 087.03^2）
脑/（ng·mg^-1）	48.25±22.41	51.73±41.92^1）	39.46±18.21
右下肢肌肉/（ng·mg^-1）	119.20±134.50	109.02±62.55	100.60±53.33^1）

组织	1/2LD50	LD50	2LD50
心血/（ng·mL^-1）	35.51±14.51^1）	70.40±44.34^1）	205.31±79.88
心肌/（ng·mg^-1）	37.22±12.69^1）	102.51±80.01	281.02±125.94
肝/（ng·mg^-1）	84.76±63.17	191.07±147.95	821.80±527.51^2）
脾/（ng·mg^-1）	-	45.68±29.92^1）	191.81±115.76
肺/（ng·mg^-1）	37.98±15.95^1）	107.97±113.80	250.19±109.38
肾/（ng·mg^-1）	395.66±167.14	798.11±506.41	2 646.55±1 087.03^2）
脑/（ng·mg^-1）	48.25±22.41	51.73±41.92^1）	39.46±18.21
右下肢肌肉/（ng·mg^-1）	119.20±134.50	109.02±62.55	100.60±53.33^1）

器官	0 h	12 h	24 h	48 h	72 h
心血/（ng·mL^-1）	1 388.64±61.21	1 272.66±775.70	715.98±35.40	616.98±101.28	660.54±127.28
心肌/（ng·mg^-1）	616.07±279.50	1 211.85±554.54	1 178.78±671.67	725.69±163.45	1 057.94±315.70
肝/（ng·mg^-1）	2 189.86±755.28	4 068.44±719.49^1）	1 989.78±1 210.59	1 798.95±338.33	721.50±337.87
脾/（ng·mg^-1）	586.84±295.48	405.79±62.18	270.30±192.85	349.25±142.71	469.96±138.98
肺/（ng·mg^-1）	832.68±397.52	2 567.02±827.59	1 898.23±529.35	1 283.39±171.60	1 709.12±235.55
肾/（ng·mg^-1）	8 183.21±1 483.04	7 666.93±861.03	9 372.31±4 825.05	6 027.35±2 354.22	4 674.76±578.87
脑/（ng·mg^-1）	86.91±20.48	170.37±113.26	187.26±70.66	148.45±83.48	281.15±124.88
右下肢肌肉/（ng·mg^-1）	291.81±76.29	410.73±174.07	350.22±284.09	317.90±134.01	354.52±94.41

器官	0 h	12 h	24 h	48 h	72 h
心血/（ng·mL^-1）	1 388.64±61.21	1 272.66±775.70	715.98±35.40	616.98±101.28	660.54±127.28
心肌/（ng·mg^-1）	616.07±279.50	1 211.85±554.54	1 178.78±671.67	725.69±163.45	1 057.94±315.70
肝/（ng·mg^-1）	2 189.86±755.28	4 068.44±719.49^1）	1 989.78±1 210.59	1 798.95±338.33	721.50±337.87
脾/（ng·mg^-1）	586.84±295.48	405.79±62.18	270.30±192.85	349.25±142.71	469.96±138.98
肺/（ng·mg^-1）	832.68±397.52	2 567.02±827.59	1 898.23±529.35	1 283.39±171.60	1 709.12±235.55
肾/（ng·mg^-1）	8 183.21±1 483.04	7 666.93±861.03	9 372.31±4 825.05	6 027.35±2 354.22	4 674.76±578.87
脑/（ng·mg^-1）	86.91±20.48	170.37±113.26	187.26±70.66	148.45±83.48	281.15±124.88
右下肢肌肉/（ng·mg^-1）	291.81±76.29	410.73±174.07	350.22±284.09	317.90±134.01	354.52±94.41

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