Abstract: It was presented that the results of EsD typing of Chinese population in Guangzhou area using agarose gel eleetrophoresis.Among 600 unrelat- ed healthy individuals tested,the numbers of EsD 1-1,EsD 2-1 and EsD 2-2 types were 163(27%),303(50.5%)and 135(22.5%),respectively, The gene frequencies of EsD~1 and EsD~2 were 0.5225 and 0.4775.It was consistent with Hardy-Weinberg equilibrium.The period of storage of bloodstains which could be typed correctly lasted 2 weeks from May through July in Guongzhou.The blood samples which were left in room condition and decaied naturally could be typed for 9 days.