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28 February 1989, Volume 5 Issue 1 Previous Issue    Next Issue
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Analysis of Band Types of Human and Animal Hemoglobin before and after Treatment with PCMB by Isoelectric Focusing WANG BAOJIE CHEN DONGQI JIA JINGTAO (FORENSIC SEROLOGY DEPARTMENT;FACULTY OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY) 1989, 0(1): 1-6+18.  Abstract ( 2059 )   Hemolysates of 41 kinds of animals including human,fetal and monkey's one,were studied by isoelectric focusing on PAG Plate (pH3.5-9.5).Species featuces of Hb band pattern,ability for identification and alternation of Hb band pattern after treatment with P-Chloromercuribenzonate (PCMB) as well as its influence on the species identification of Hb were disscused as well.Approaching to the application of IEF for identifying species gave the following results:Class of animals can be identified hy characteristic distri- bution of the band pattern;Human Hb may be distinguished from that of other animals but monkey's one of which band types somewhat difficultly discriminated because of similar band type,Differences of band patterns are also shown between animals,which may be used for identification.Aftry treatment with PCMB,both human and monkey's Hb are broken down to and β subunit.The different PI value of α subunitin both human and monkey's Hb can be used for identification and much easily to distinguish human Hb from that of the other animals. Related Articles | Metrics

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Detection of Erythrocyte Isoenzymes EsD and GLOI Phenotypes Using a Mixed Starch Agarose Gel Electrophoresis DA JIEN WANG JIENWEN CHEN QIAOFEN (DEPARTMENT OF FORENSIC MEDICINE;(?)ANGJING MEDICAL COLLEGE) 1989, 0(1): 7-9.  Abstract ( 1919 )   This paper shows the detection of the phenotypes of isoenzymes EsD and GLOI for fresh blood cells using the same mixed starch agarose gel electrophoresis and Tris-maleic acid buffer system at pH7.4.Among 302 fresh blood samples,the EsD gene frequencies were:EsD~1=0.641,EsD~2= 0.359;Among 401 fresh blood samples,the GLOI gene frequencies:were: GLOI~1=0.1895,GLOI~2=0.8105,It is useful to paternity,test in Nangjing area. Related Articles | Metrics

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A Study of Specificity of Human ABH Antigen and Use of Species Identification CHEN JIAN (INS.OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;PRC) 1989, 0(1): 10-14.  Abstract ( 2575 )   12 monoclonal antibody (McAb) include 5 anti-A,5 anti-B,1 anti- H and 1 anti-A+B,and polyclonal human serum anti-A andanti-B were test in parallel with more than 20 animal erythrocytes include pan troglodyles pango pygmaeus,monkey,cynoeephalus balouin etc.We found some McAb can discriminate all the measuing animals,that make it possi- ble to use McAb in species identification.McAb anti-A,anti-B and anti-H using in forensic sicence have two adventages:a)high specifi- city,some of them can discriminate primate animals.b) it can finish species identification and ABe groups measure in one procedure. Related Articles | Metrics

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The Studies on the Distribution of EsD Phenotypes of Chinese Population in Guangzhou Area and EsD Phenotyping in Bloodstains WU XINYAO LEE JIANJING KUO JINGYUAN KUO YINGSHUI TAI JENG (DEAPRTMENT OF FORENTIC BIOLOGY;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES;GUANGZHOU) 1989, 0(1): 15-18.  Abstract ( 1920 )   It was presented that the results of EsD typing of Chinese population in Guangzhou area using agarose gel eleetrophoresis.Among 600 unrelat- ed healthy individuals tested,the numbers of EsD 1-1,EsD 2-1 and EsD 2-2 types were 163(27%),303(50.5%)and 135(22.5%),respectively, The gene frequencies of EsD~1 and EsD~2 were 0.5225 and 0.4775.It was consistent with Hardy-Weinberg equilibrium.The period of storage of bloodstains which could be typed correctly lasted 2 weeks from May through July in Guongzhou.The blood samples which were left in room condition and decaied naturally could be typed for 9 days. Related Articles | Metrics

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Study on the Typing of EAP with PAGIF and Distributon of EAP in Shanghai LIU YAN HE GENLAN (INSTITUTE OF FORENSIC SCIENCE;MINISTRY OF JUSTICE;PRC) 1989, 0(1): 19-24.  Abstract ( 1993 )   A method was described for determining erythrocyte acid phosphatase (EAP) phenotypes from fresh human blood and bloodstain by thin-layer polyacrylamide gel isoelectric focusing (PAGIF),obtaining arrow,even, clear isoenzyme bands Some of the advantages of thin-layer PAGIF were increased resulation and dried bloodstain for twomonthes can be detected. Using this method,We detected the EAP phenotypes from 283 unrelated individuals in Shanghai.The results were as follows:EAP A 4.24%,EAP BA 28.98%,EAP B 66.78%;p~a=0.187,p~b=0.813;Did not find p~c.The discriminating power (DP)of EAP was 0.47. Related Articles | Metrics

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A Study on Post Mortem Changes of Esterases in Rat Liver YU JIAMEI WANG ERWEN (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;PRC) 1989, 0(1): 25-28.  Abstract ( 1971 )   This study was shown that the Esterase isoenzyme in rat liver would change after its death,especially within 6 hours.The changes of Ester- ases in rat liver appeared regularly.It was found that some ons decreas- ed.and some ones increased.We thought this method was very useful in determining the time of death. Related Articles | Metrics

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The Different Human Spermatozoa HLA-AB Typing Identified by Two Fluorescence Technique CHENG DALIN LIU ZUBO (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICCE;PRC) 1989, 0(1): 29-32.  Abstract ( 1972 )   The HLA-AB typing of human spermatozoa identified by two colour fluorescence technique is reported in three cases.with the acridine orange and the ethidium bromide as stains,the human spermatozoa affected by HLA-AB antibody appeared two distinct colour fluorescence: the orange-red indicated dead spermatozoa earring the relevant HLA-AB antigen and jade green living ones noncarrying the antigen. Applied to the identification in disputed paternity,the HLA-AB typ- ing of human spermatozoa as haploid would raise the nonfatherexclusion probability (NFEP) of the assaying system,and as well as raise the paternity index (PI) and the relative chance of paternity (RCP). Related Articles | Metrics

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Studies on the Disperse Dyes with Differential Scanning Calorimetry YAO ZHONGDONG YE MINGHUA (INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;PRC) 1989, 0(1): 40-42.  Abstract ( 2013 )   The identification and recognization of textile fibers by using differ- ential scanning calorimetry (DSC) technique is deteiled.In this study we mainly applied the DSC to identify the fibers dyed with a type of dye in different concertration and identify a kind of dye in different type.The results are showed that:a,the DSG method can be used to identify the dyes in the same or similar colour but in different types,bs the DSC curves of dyed fibers appeared not only the fibers special peaks but also the new special peak that dyes act on fibers. Related Articles | Metrics