Abstract: Blood samples were analyzed for ethanol,aceta- dehyde and acetate by head space gas chroma- tography using a porapak S column.Acetate was esterified to methyl acetate simply by the addition of acidified methanol.After the oral administration of ethanol to two healthy human subjects,the concentration versus time profiles of blood ethanol, acetadehyde and acetate were determind.The ad- vantage of the method is its speed and simplicity.