Abstract: A reveal-phase HPLC method for determination ofphenothiazines and tricyclin antidepressantS in wholeblood was described. In this paper, a model 1090HPLC with DAD and a zorbax ODS column wasused. The mobile phase was methanol: water: tritelyamine (75:24.7:0.3) with PH 7.5. Cyproheptadinewas used as internal standard in this method. Bloodsamples were extracted with the solid-phaseextraction method and the liquid-liquid method. Thismethod is suitable in forensic toxicology analysis forbasic drugs.

Key words: HPLC, phenothiazines, tricyclin, forensic toxicology