›› 1998, Vol. 0 ›› Issue (2): 87-88+1.

• 论文 • Previous Articles     Next Articles

Extraction and amplification of DNA from formalin-fixed human tissues

LI LI. QUE TINGZHI, LIN YUAN, GONG YUCHANG (INSTITUTEOF FORENSIC SCIENCES, MINISTRY OF JUSTICE, SHANGHAI200063)   

  • Online:1998-04-25 Published:1998-04-28

Abstract: A method was developed for the extraction of DNAfrom formalin-fixed human tissues prepared for routinehispathological examination. The extracted DNA wasnot intact. By increasing the formalin fixation time thenecessary amount of intact DNA decreased. Theprocedure reported here made the final product suitablefor quantitative and qualitative analysis and for DNAtyping by PCR technique.

Key words: formalin-fixed tissue, extraction of DNA, PCR