Abstract: A GC method was established for qualitative and quantitative determination of chloroquine in human urine. The chloroquine is extracted from 2ml urine with 2 x 2ml cyclohexame: chloroform (8: 2). The retention time of chloquine is9.44min. The linear of quantitation is 0 - 50μg /ml. Therecovery of the procedure in 25μg g/ml is 87 .0%, CV = 7.9% (n = 5). The limit of the determination is 200ng/ml.The method can also be used in blood. A case is reportedhere. In that, we detected chloroquine in urine and blood.The quantitation of chloroquine in urine and blood is 0. 475mg/ml and 3 .68μg/ml, respectively. A metabolite of chloroquine - desethylchloroquine is also found in the urine.

Key words: chloroquine, urine, GC