Study of the mechanism of cultured neuron injury mediated by nitric oxide during hypoxia and oxidative stress

Abstract

Abstract: Objective To study the mechanisms of cultured neurons injury mediated by nitric oxide and free oxygen radicle during hypoxia and oxidative stress.Methods The cultured newborn rat neurons were treated with hypoxia, H_2O_2 and pretreated superoxide dismutase (SOD) respectively. We examined the content of NO, malonaldehyde (MDA), lactate dehydrogenase (LDH) and SOD in cultured supernatant.Results Comparing with that of control group, the content of NO, LDH, MDA increased and the content of SOD decreased in hypoxia group and H_2O_2 group. The content between NO and SOD showed the negative correlation. Administration of 200 U/ml SOD before oxidative stress could efficiently decrease the release of NO, LDH and MDA in neurons. The content of NO, LDH and MDA manifested in postive correlation in each group.Conclusion Hypoxia and oxidative stress increased NO production which strengthen neurons injury induced by free radicle. SOD played an important role in elimination of free oxygen radicals and protecting neurons from injury by NO. [

Key words: hypoxia, oxidative stress, nitric oxide, free oxygen radicle, neuron injury

GUAN PENG;AI XIAO－ MAN;YU RU－ TONG;ET AL. (. COLLEGE OF FORENSIC MEDICINE;WEST CHINA UNIVERSITY OF MEDICAL SCIENCE;CHENGDU 00;. LABORATORY IN BEIJING HOSPITAL;BEIJING 000;.DEPARTMENT OF NEUROSURGERY IN FIRST HOSPITAL;WEST CHINA UNI. Study of the mechanism of cultured neuron injury mediated by nitric oxide during hypoxia and oxidative stress[J]. , 2001, 0(2): 79-81+8.

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Study of the mechanism of cultured neuron injury mediated by nitric oxide during hypoxia and oxidative stress

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