Abstract: The methods of morphology, cytology, sex hormone detection, DNA recobination and polymerase chain reaction (PCR) were described for sex identification. It was summarized that the morphology is better for skeleton identification; the amelogenin detection is most suggestible for the human tissues from which, enough quantity of genomic DNA is available. While sex chromatin examination gives only lower positive rates. The examination of sex hormone only be used for bloodstain sample.Because of the requirement of complex instruments and radio－ labeled probe, DNA molecular hybridization is now rarely used.

Key words: sex identification, sex chromatin, sex hormone, molacular hybridization, PCR, amelogenin