Abstract: Objective To determine the changes of intercellular adhesion molecule-1 after traumatic brain injury. Methods Use the fluid-percussion model to inflict rat brain and take the samples at 2?4?8?12?24 hours after brain injury. The samples were studied by immunohistochemistry method combined with image analysis by MIAS image analysis system. Result The expression of ICAM-1 upregulates at 2 hours, reaches peak at 12 hours and falls down at 24 hours after brain injury. Conclusion The changes of ICAM-1 in brain can provide a backup for estimation of injury time and survival period after brain injury which sustained within 24 hours.

Key words: brain injury, ICAM-1, immunohistochemistry, image analysis