The expression of FN-Ⅲcs in the wound healing and its relationship with injury time

Abstract

Abstract: Objective To research the expression of alternative splicing segment of fibronectin-Ⅲcs in incised wound of skin, to offer the experimental data for early injury time judgement.Methods Using in situ hybridization with DIG labeled anti sense RNA probe, the expression of FN Ⅲcs domain was detected in both human and rat skin incised wound. Results (1) The expression of FN Ⅲcs improved from the time of 30 min after injury, reached peak at 6h and then reduced gradually.(2) The positive expression cells were mainly distributed in hair follicles, sebaceous glands and endothelium, however, in human beings they were basement cells of epidermis. Conclusion The expression of FN Ⅲcs domain would be a supposed useful criteria for early injury time judgement.

Key words: FN Ⅲtype connective segment, in situ hybridization, judgement of early injury time

JI YAN;ZHAO ZI-QIN;GU YUN-JU;ET AL.(DEPARTMENT OF FORENSIC MEDICINE OF FUDAN UNIVERSITY;SHANGHAI 000). The expression of FN-Ⅲcs in the wound healing and its relationship with injury time[J]. , 2002, 0(1): 4-6.

[1]	PEI MING;SUN JUN-HONG;GAO CAI-RONG(.SCHOOL OF FORENSIC MEDICINE;SHANXI MEDICAL UNIVERSITY;TAIYUAN 0000;CHINA;.QUANSHAN BRANCH OF XUZHOU PUBLIC SECURITY BUREAU;XUZHOU 00;CHINA). Changes of ICAM-1 mRNA Expression in Rats' Skeletal Muscle after Contusion [J]. , 2009, 0(3): 161-163.
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[5]	MA MENG-YUN;XU XIAOHU (SHENZHEN CRIMINAL SCIENCE & TECHNOLOGY INSTITUTE;SHENZHEN 00;CHINA;COLLEGE OF MEDICAL SCIENCE;SHANTOU UNIVERSITY;SHANTOU 0;CHINA). Experiment study of c-fos expression on myocardial acute ischemia/reperfusion injury in rats [J]. , 2003, 0(2): 65-67+1.
[6]	XIAO JUN-HUI;LI YONG-HONG(DEPARTMENT OF FORENSIC PATHOLOGY;WANNAN MEDICAL COLLEGE;WUHU00). The expression of HSP70mRNA after myocardial ischemia and reperfusion injury by in situ hybridization [J]. , 2002, 0(4): 199-200+.
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The expression of FN-Ⅲcs in the wound healing and its relationship with injury time

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