A analysis of ELISA on the time-related expression of IL-2 and TNF-a during the healing process of wound in rat skin

Abstract

Abstract: Objective In order to explore the relationship between the expression change of cytokines and the wound age during the healing process of rats skin wound. Methods A assay of ELISA were performed on intravital skin wounds(after incision 0.5-168 h) to detect the dynamics expression level of IL-2, TNF-a. Results The level of IL-2 and TNF-a increased at 0.5 h after wounding, then got to a peak at 3 h and 1 h after injure respectively. Rebound of TNF and IL-2 levels were shown at 48 h after wounding, and both cytokine levels were inclined to elevating between 72h and 168h after wounding. Conclusion The cytokine level changes suggest they have a time-related expression during wounds healing process.

Key words: cytokines, expression of IL-2 and TNF-a, ELISA, wound age estimation

WANG HUI-JUN;DING YUN-CHUAN (DEPARTMENT OF PATHOLOGY;THE FIRST MILITARY MEDICAL UNIVERSITY;GUANGZHOU 0). A analysis of ELISA on the time-related expression of IL-2 and TNF-a during the healing process of wound in rat skin[J]. , 2003, 0(1): 10-12.

[1]	Yan-ru XI, Yuan-xin LIU, Na FENG, Zhen GU, Jun-hong SUN, Jie CAO, Qian-qian JIN, Qiu-xiang DU. Application of Linear Regression Model of Gpnmb Gene in Rat Injury Time Estimation [J]. Journal of Forensic Medicine, 2022, 38(4): 468-472.
[2]	Jian CHENG, Long-long SUO, Lin-lin WANG, Rui ZHAO, Da-wei GUAN. Application Prospect of MicroRNA in Skin Wound Age Estimation [J]. Journal of Forensic Medicine, 2021, 37(6): 841-846.
[3]	Xue-shi CHEN, Jun CHU, Li-jun YANG, Tao WANG, Lu-yang TAO. Application of Immunohistochemistry and Special Staining Technique in Forensic Traumatic Pathology Identification [J]. Journal of Forensic Medicine, 2021, 37(5): 666-672.
[4]	YAO Yi HUANG Jun-jie, JIN Xin,et al.. Function of IL-33 in Wound Age Estimation of Skin Wounds in Mice [J]. 法医学杂志, 2020, 36(2): 192-198.
[5]	WEN Shu-heng, TIAN Zhi-ling, ZHANG Miao，et al.. Relationship between Changes of Pericyte Number and Wound Age during Repair of Skeletal Muscle Contusion in Mice [J]. 法医学杂志, 2019, 35(3): 267-272，279.
[6]	GUO Xiang-shen, WEN Shu-heng, DONG Wen-wen，et al.. Expression of Nrf2 in Different Cells after Human Cerebral Cortex Contusion [J]. 法医学杂志, 2019, 35(3): 273-279.
[7]	CHEN Jing-wei, WANG Peng-fei, ZHANG Meng-zhou1，et al.. Relationship between Expression Changes of CB2R and Wound Age of Brain Contusion in Mice [J]. 法医学杂志, 2019, 35(2): 136-142.
[8]	ZHAO Jian-xin, JIN Xin, HUANG Jun-jie，et al.. Time-dependent Expression and Distribution of AChE during the Skin Incised Wound Healing in Mice [J]. 法医学杂志, 2019, 35(2): 143-148.
[9]	HUANG Jun-jie, YAO Yi, XIA Chong-Jian，et al.. Relationship between the Number of Neutrophils and Myofibroblasts during Diabetic Wound Healing and Wound Age [J]. 法医学杂志, 2019, 35(2): 149-153.
[10]	LIU Ran, GE Lu-zou, ZHANG Hai-dong，et al.. Expressions of TGF-β1 and EⅢA-FN after Rat Skeletal Muscle Contusion and Wound Age Estimation [J]. 法医学杂志, 2019, 35(2): 154-159.
[11]	LU Han-lin, DANG Li-hong, LI Na, et al.. Combination of mRNA of Repair-related Genes in Rat Skeletal Muscles for Wound Age Estimation [J]. 法医学杂志, 2019, 35(2): 160-165.
[12]	LIU Qi-qing, GUO Hong-min, WANG Lei，et al.. Wound Age Estimation by Neutrophil Migration Distance#br# [J]. 法医学杂志, 2019, 35(2): 166-170.
[13]	DU Qiu-xiang, ZHU Xi-yan, DONG Ta-na，et al.. Homogeneity of Different Functional mRNA Indicators for Wound Age Estimation [J]. 法医学杂志, 2018, 34(5): 487-491.
[14]	ZHANG Xiao-Hong, LIU Ji, LI Na-1，et al. Time-dependent Expression of Nrf2 Protein and its Effects on Wound Repair in Skeletal Muscle [J]. , 2018, 34(1): 18-22.
[15]	DONG TA-NA, PEI MING, LI NA, ET AL.. Relationship between Wound Age and the Expression of Fzd2 in Rats Skeletal Muscle after Contusion [J]. , 2017, 33(4): 344-347.

A analysis of ELISA on the time-related expression of IL-2 and TNF-a during the healing process of wound in rat skin

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics