Abstract: Objective To establish a new method for determination of strychnine alkaloid in biological fluids based on molecularly imprinted polymers. Methods A strychnine molecularly imprinted monolithic column was prepared by in-situ molecularly imprinted technique. The polymer was filled to a 1cm column, and a method was developed to concentrate and determine strychnine alkaloids in biological fluids. Results The limit of detection of the method was 4.9 ng, and the recoveries were more than 92%. The relative standard deviations were smaller than 6.59%. The linear correlation coefficients of standard curves were 0.999 1 and 0.996 6 respectively. This method was applied to concentrate and determine strychnine in plasma and urine of poisoned rabbit. Conclusion The new method could concentrate and simultaneously determine strychnine alkaloids in biological fluids, and it was applied to forensic toxicological analysis.

Key words: molecularly imprinted technique, strychnine, accumulation