Forensic Applications of the Sequencing of Mitochondrial DNA Cytochrome Oxidase SubunitⅠGene for Sarcosaphagous Flies (Diptera) in Huhhot and Dunhuang District

›› 2005, Vol. 0 ›› Issue (2): 100-103+.

Forensic Applications of the Sequencing of Mitochondrial DNA Cytochrome Oxidase SubunitⅠGene for Sarcosaphagous Flies (Diptera) in Huhhot and Dunhuang District

CAI JI-FENG1, DONG JIAN-GUO2, LIU MIN1, ET AL. (1.WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE, SICHUAN UNIVERSITY, CHENGDU 610041,CHINA; 2.DEPARTMENT OF PATHOLOGY, SCHOOL OF PRECLINICAL MEDICINE, SHANGHAI SECOND MEDICAL UNIVERSITY, SHANGHAI 200

Online:2005-04-25 Published:2005-04-28

Abstract

Abstract: Objective To solve the problems of identification of Sarcosaphagous flies and their larvae, pupas and eggs. Methods Sarcosaphagous Flies (Diptera) Samples were collected on the corpses of rabbits in the Huhhot district and a pig in the Dunhuang district. A 278bp region in the cytochrome oxidase subunitⅠ (COⅠ) gene in mtDNA was analysed by DNA sequencing, A neighbour-jioning tree using the Tamura and Nei model of nucleotide substitution was also constructed using the MEGA2.1 package. Results A 278 base pairs region of the gene for COⅠencoding region of mtDNA of above all samples was showed less than 1% sequence divergence within species and about 3% divergence between species. Conclusion It is an effective, easy and accurate method to be used for identification of these Sarcosaphagous Flies (Diptera) to species group by sequencing the 278 base pairs region of the COⅠencoding gene of mtDNA.

Key words: Sarcosaphagous flies, species identification, mtDNA, COⅠ

CAI JI-FENG;DONG JIAN-GUO;LIU MIN;ET AL. (.WEST CHINA SCHOOL OF PRECLINICAL AND FORENSIC MEDICINE;SICHUAN UNIVERSITY;CHENGDU 00;CHINA;.DEPARTMENT OF PATHOLOGY;SCHOOL OF PRECLINICAL MEDICINE;SHANGHAI SECOND MEDICAL UNIVERSITY;SHANGHAI 00. Forensic Applications of the Sequencing of Mitochondrial DNA Cytochrome Oxidase SubunitⅠGene for Sarcosaphagous Flies (Diptera) in Huhhot and Dunhuang District[J]. , 2005, 0(2): 100-103+.

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Forensic Applications of the Sequencing of Mitochondrial DNA Cytochrome Oxidase SubunitⅠGene for Sarcosaphagous Flies (Diptera) in Huhhot and Dunhuang District

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