Detection of γ-Hydroxybutyrate(GHB) in Beverages

Abstract

Abstract: Objective To establish an analytical method for the determination of GHB in beverages using GC/MS and LC/MS/MS.Methods After beverage samples with GHB-d6 as the internal standard were extracted with ethyl acetate,then the extracts were derivatized with N,O-bis(trimethylsilyl) trifluoroacetamide(BSTFA) ,at last the derivateized extracts analyzed by gas chromatography-mass spectrometry.After beverage samples with GHB-d6 as the internal standard were diluted by mobile phase then directly analyzed by LC/MS/MS.Results The limit of detection was 0.2 μg/mL and both relative standard deviations for between-day and within-day assays were < 8.54% in GC/MS.The limit of detection was 2 μg/mL and both relative standard deviations for between-day and within-day assays were <8.62% in LC/MS/MS.Conclusion These methods of qualitative and quantitative analysis were found to be sensitive,accurate,rapid and suitable for the forensic toxicology to test of GHB in real cases.

Key words: γ-Hydroxybutyrate(GHB), beverage, GC/MS, LC/MS/MS

LIU WEI;SHEN MIN;XIANG PING;BU JUN;WU HE-JIAN(INSTITUTE OF FORENSIC SCIENCES;MINISTRY OF JUSTICE;P.R.CHINA;SHANGHAI 000;CHINA). Detection of γ-Hydroxybutyrate(GHB) in Beverages[J]. , 2007, 0(2): 120-122+.

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Detection of γ-Hydroxybutyrate(GHB) in Beverages

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