Abstract: Objective To investigate the polymorphism of C-stretch in mitochondrial DNA hyper-variant region and the values in forensic science.Methods The gene frequency were detected in such two loci of nt16180 and nt310 by using PCR-sequencing technique with two pair of primers according to the reference.Results The length of PCR products were 807 bp and 962 bp respectively.Seven genotypes in each locus were detected.AAAACCCCCTCCCC allele amounted to 87.72% of all nt16180 genotype,while AAAACCCCCCCCCCCCC was firstly reported in Han population.CCCCCCCCTCCCCCC allele amounted to 60.53% of all nt310 genotype.15 haplotypes were detected among 114 samples.The GD value was 0.630 9.The AAAACCCCCTCCCC-CCCCCCCCTCCCCCC haplotype was the most high frequency and amounted to 57.89%.Conclusion The obtained basic genetic data in two loci of nt16180 and nt310 of mtDNA will be useful for forensic science.

Key words: mitochondrial DNA, hyper variant region, C-stretch, sequence-length polymorphism, sequencing