Cross-Platform Application of DNA Methylation Age Estimation Model in Eastern Chinese Han Population

doi:10.12116/j.issn.1004-5619.2022.520405

Abstract

Abstract:

Objective To evaluate the forensic application value of an age estimation model based on DNA methylation in eastern Chinese Han population， and to provide a theoretical basis for exploring age estimation models suitable for different detection platforms. Methods According to the 6 age-related methylation sites in the published blood DNA methylation age estimation models of Chinese Han population， the DNA methylation level of 48 samples was detected by pyrosequencing and next-generation sequencing （NGS）. After submitting DNA methylation levels to the age estimation model， the DNA methylation ages were predicted and compared with their real ages. Results The 6 DNA methylation sites in both detection techniques were age-related， with an R² of 0.85 and a median absolute deviation （MAD） of 4.81 years when using pyrosequencing； with an R² of 0.84 and MAD of 4.41 years when using NGS. Conclusion The blood DNA methylation age estimation model can be used under pyrosequencing and multi-purpose regional methylation enrichment sequencing technology based on NGS and it can accurately estimate the age.

Key words: forensic genetic, epigenetics, age estimation, DNA methylation, pyrosequencing, next-generation sequencing

CLC Number:

Zi-wei WANG, Cheng-tao LI, Xi-ling LIU. Cross-Platform Application of DNA Methylation Age Estimation Model in Eastern Chinese Han Population[J]. Journal of Forensic Medicine, 2023, 39(5): 441-446.

Figures/Tables 6

References 21

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年龄段/岁	男性	女性	合计
合计	24	24	48
3~20	6	6	12
>20~39	6	6	12
>39~60	6	6	12
>60	6	6	12

年龄段/岁	男性	女性	合计
合计	24	24	48
3~20	6	6	12
>20~39	6	6	12
>39~60	6	6	12
>60	6	6	12

CpG位点	CpG ID	基因	位置	扩增片段长度/bp
CpG1	-	ITGA2B	chr17：44，390，358	153
CpG2	cg14361627	KLF14	chr7：130，734，355	99
CpG3	cg06639320	FHL2	chr2：105，399，282	229
CpG4	cg04208403	ZNF423	chr16：49，491，896	113
CpG5	cg02228185	ASPA	chr17：3，476，273	104
CpG6	cg19283806	CCDC102B	chr18：68，722，183	197

CpG位点	CpG ID	基因	位置	扩增片段长度/bp
CpG1	-	ITGA2B	chr17：44，390，358	153
CpG2	cg14361627	KLF14	chr7：130，734，355	99
CpG3	cg06639320	FHL2	chr2：105，399，282	229
CpG4	cg04208403	ZNF423	chr16：49，491，896	113
CpG5	cg02228185	ASPA	chr17：3，476，273	104
CpG6	cg19283806	CCDC102B	chr18：68，722，183	197

CpG位点	焦磷酸测序	NGS	SNaPshot^[11]
CpG1	-0.705 7	-0.726 1	-0.666 1
CpG2	0.837 7	0.831 3	0.818 2
CpG3	0.515 2	0.915 4	0.789 2
CpG4	-0.859 0	-0.889 7	-0.535 5
CpG5	-0.770 3	-0.763 9	-0.628 6
CpG6	-0.848 4	-0.839 4	-0.870 4