Detecting ABO Blood Type of Bloodstain with Fluorescent Antibody Method

›› 2007, Vol. 0 ›› Issue (2): 127-129+.

Detecting ABO Blood Type of Bloodstain with Fluorescent Antibody Method

WANG RUI-HENG1,YAN LI-QIANG2,YU WEI-JIAN3,LIU XIAO-WEI4,QU SHU-XIAN5(1.LAW SCHOOL OF LIAONING NORMAL UNIVERSITY,DALIAN 116029,CHINA;2.LIAONING POLICE ACADEMY,DALIAN 116033,CHINA;3.DALIAN RED CROSS BLOOD CENTER,DALIAN 116001,CHINA;4.FIRST HOSPITAL DALIAN M

Online:2007-04-25 Published:2007-04-28

Abstract

Abstract: Objective To explore the advantage and feasibility of fluorescent antibody method for detection of blood type in biological material.Methods According to theory of specific binding of antigen and antibody,at first the anti-A monoclonal antibody(MA) and anti-B MA were labeled with the fluorescent,then fluorescent-labeled antibodies(FLA) were bound with corresponding biological material(such as bloodstain) in the optimum condition,finally the ABO blood type of bloodstain was determined under microscope fluorescent.Results The fluorescent antibody method is highly sensitive,accurate and simple.Conclusion The fluorescent antibody method is an accurate and reliable method for detection of ABO blood type in biological material.

Key words: fluorescent antibody method, bloodstain, ABO blood type

WANG RUI-HENG;YAN LI-QIANG;YU WEI-JIAN;LIU XIAO-WEI;QU SHU-XIAN(.LAW SCHOOL OF LIAONING NORMAL UNIVERSITY;DALIAN 0;CHINA;.LIAONING POLICE ACADEMY;DALIAN 0;CHINA;.DALIAN RED CROSS BLOOD CENTER;DALIAN 00;CHINA;.FIRST HOSPITAL DALIAN M. Detecting ABO Blood Type of Bloodstain with Fluorescent Antibody Method[J]. , 2007, 0(2): 127-129+.

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Detecting ABO Blood Type of Bloodstain with Fluorescent Antibody Method

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