The Correlation Between the DNA Content in Human Thyroid Follicular Epithelial Cells and the Postmortem Interval

Abstract

Abstract: Objective To study the relationship between the DNA content in follicular epithelial cells of the human thyroid and postmortem interval(PMI) .Methods Changes of the DNA content in thyroid follicular epithelial cells at different PMI were determined by Methyl Green-Pyronin(MGP) stain combined with an image analysis technique.Results The average DNA content in the thyroid follicular epithelial continued to decrease with increased PMI.The coefficient of determination for DNA content with average gray,aimed area,aimed area ratio and positive index in follicular epithelial cells were 0.960,0.987,0.988 and 0.990,respectively.Conclusion There is an apparent correlation between the average DNA content of follicular epithelial cells and the PMI.MGP stain combined with an image analysis technique seems to be a useful means to study the degradation of DNA in thyroid follicular epithelial cells.

Key words: postmortem interval(PMI), methyl green-pyronin stain, thyroid follicular epithelial cells, DNA degradation

REN GUANG-MU;JING CAI-LIANG;LIU JI;WANG YA-FANG;WANG YING-YUAN(SCHOOL OF FORENSIC MEDICINE;SHANXI MEDICAL UNIVERSITY;TAIYUAN 0000;CHINA). The Correlation Between the DNA Content in Human Thyroid Follicular Epithelial Cells and the Postmortem Interval[J]. , 2007, 0(2): 92-93+9.

[1]	JIE YA-LING, LI LU, SHAO CHENG-CHEN, ET AL.. Application of the Peak Area Ratio of STR Loci to Amelogenin Locus in the Estimation of DNA Degradation [J]. , 2016, 32(2): 105-108.
[2]	FANG CHAO, LIN XIAO-HONG, JING HUA-LAN. DNA Degradation of Porcine Retinal Cells for Estimation of Postmortem Interval [J]. , 2010, 26(4): 250-252.
[3]	ZHENG JI-LONG;LI XIAO-NA;ZHANG XIAO-DONG;NIU QING-SHAN(.SCHOOL OF FORENSIC MEDICINE;CHINA MEDICAL UNIVERSITY;SHENYANG 000;CHINA;.DEPARTMENT OF FORENSIC MEDICINE;CHINA CRIMINAL POLICE UNIVERSITY;SHENYANG 00;CHINA;.COLLEGE OF BASIC MEDICAL S. DNA Degradation in Nucleolus of Skeletal Muscle, Heart, Liver, Kidney and Brain in Mice after Death [J]. , 2010, 0(3): 161-164.
[4]	HAO LU-GUI;DENG SHI-XIONG;ZHAO XIN-CAI(.DEPARTMENT OF FORENSIC MEDICINE;CHONGQING MEDICAL UNIVERSITY;CHONGQING 000 CHINA;.CHONGQING TRAFFIC ADMINISTRATION BUREAU;CHONGQING 000 CHINA). Recent Advancement in Relationship between DNA Degradation and Postmortem Interval [J]. , 2007, 0(2): 145-147.
[5]	ZHEN JI-LONG;ZHANG XIAO-DONG;NIU QIN-SHAN(DEPARTMENT OF FORENSIC MEDICINE OF CHINESE CRIMINAL COLLEGE;SHENYANG 00;CHINA). Relationship between the Postmortem Interval and Nuclear DNA Changes of Heart Muscular Cells in Mice [J]. , 2006, 0(3): 173-176.
[6]	LUO GUANG-HUA;CHEN YU-CHUAN;CHENG JIAN-DING;WANG JANG-FENG;GAO CUI-LIAN (.GUANGDONG JUSTICE POLICE VOCATIONAL COLLEGE;GUANGZHOU 00;CHINA;.GUANGDONG POLICE COLLEGE;GUANGZHOU 0;CHINA;.DEPARTMENT OF FORENSIC PATHOLOGY;ZHONGSHAN MED. Relationship between DNA Degradation and Postmortem Interval of Corrupt Corpse [J]. , 2006, 0(1): 7-9+97.
[7]	WU RONG-QI;ZHAO ZI-QIN;SHEN YI-WEN;JIA JIAN-ZHANG (DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL COLLEGE;FUDAN UNIVERSITY;SHANGHAI 000;CHINA). Estimation of Postmortem Interval Using Myofibril Fragmentation Index [J]. , 2006, 0(1): 10-11+1.
[8]	LONG REN;WANG WEI-PING;XIONG PING;ET AL. (.DEPARTMENT OF FORENSIC MEDICINE;NANHUA UNIVERSI-TY;HENGYANG 00;CHINA;.PEOPLE′S COURT OF HENGYANG;HENGYANG 00;CHINA). Correlation between PMI and DNA Degradation of Costicartilage and Dental Pulp Cells in HumanBeing [J]. , 2005, 0(3): 174-176.
[9]	CHEN XIN;SHEN YI-WEN;GU YUN-JU;ET AL. (.SHANGHAI INSTITUTE OF CRIMINAL SCIENCE & TECHNOLOGY;SHANGHAI 000;CHINA;. DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL COLLEGE;FUDAN UNIVERSITY;SHANGHAI 000;CHINA). The Research of Relationship between DNA Degradation and Postmortem Interval [J]. , 2005, 0(2): 115-117.
[10]	ZHAN LAN;ZHAO ZI-QIN;SHEN YI-WEN;ET AL. (DEPARTMENT OF FORENSIC MEDICINE;SHANGHAI MEDICAL COLLEGE;FUDAN UNIVERSITY;SHANGHAI 000;CHINA). Estimation of Postmortem Intervals by Using Terminal Deoxynucleotidyl Transferase [J]. , 2005, 0(2): 113-114.
[11]	SHU XI-JI;LIU-LI-JIANG;HU JIA-WEI;ET AL.(.SCHOOL OF MEDICINE﹠LIFE SCIENCES;JIANGHAN UNIVER-SITY;WUHAN00;CHINA;.THE DEPARTMENT OF PUBLIC SECURITY;WUHAN00;CHINA;.FACULTY OF FOREN-SIC MEDICINE;TONGJI MEDICAL COLLEGE;HUAZHONG UNIVERSITY OF SCI. Study on relationship between changes of geometry parameters in human spleen nuclei and the postmortem interval [J]. , 2004, 0(3): 140-142.
[12]	GONG ZHI-QIANG ;ZENG XIAN-BIN ;SUN YUE-GANG ;ET AL.(.DEPARTMENT OF FORENSIC MEDICINE;JIANGXI MEDICAL COLLEGE;NANCHANG000;.PEOPLE'S COURT OF NANCHANG;NANCHANG000;.THE CRIMINAL SCIENCE RESEARCH INSTITUTION;BUREAU OF NANCHANG. Study on the relationship between PMI and the concentration of21elements in vitreous humor of rabbit after death [J]. , 2002, 0(2): 67-69.
[13]	GONG ZHI－ QIANG;XU XIAO－ MING;ZENG XIAN－ BIN;ET AL. (DEPARTMENT OF FORENSIC MEDICINE;JIANGXI MEDICAL COLLEGE;NANCHANG 000;PEOPLE'S COURT OF NANCHANG;NANCHANG 000). Study on the relationship between PMI and the concentration of zincum and nickel in the vitreous humor of rabbit after death [J]. , 2001, 0(3): 129-131.

The Correlation Between the DNA Content in Human Thyroid Follicular Epithelial Cells and the Postmortem Interval

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 13

Recommended Articles

Metrics