Journal of Forensic Medicine

›› 2007, Vol. 0 ›› Issue (3): 199-200.

• 论文 • Previous Articles     Next Articles

Analysis of 7-Aminonitrazepam in Urine by Pentafluoropropionyl Derivatization-Gas Chromatography/Mass Spectrometry

ZHUO XIAO-CONG1, XIE YU1, CHU JIAN-XIN2(1.XIAOSHAN BRANCH OF HANGZHOU PUBLIC SECURITY BUREAU, HANGZHOU 311200,CHINA; 2.THE PEOPLE'S PROCURATORATE OF ZHEJIANG PROVINCE,HANGZHOU 310012,CHINA)   

  • Online:2007-06-25 Published:2007-06-28

Cited

2

Abstract: Objective This paper reported a new method for the analysis of 7-amnonitrazepam(7-ANIZ), the major metabolite of nitrazepam in urine by pentafluoropropionyl derivatization-GC/MS. Methods After urine sample were extracted with ethyl ether, the extracts were derivatized with pentafluoropropionic anhydride (PFPA), and then total ion current chromatograms(TIC) of the derivatives were acquired. Results The qualitative analysis of 7-ANIZ can be built by tR and m/z; The analysis of 7-ANIZ can be built by internal standard method. Conclusion The analytical method of qualitative and quantitative analysis of 7-ANIZ was sensitive and accurate.

Key words: GC/MS, pentafluoro-propionyl derivatization, urine, nitrazepam, 7-amnonitrazepam