Research Progress on Hair Metabolomics and Its Application in Forensic Science

doi:10.12116/j.issn.1004-5619.2023.331003

Abstract

Abstract:

Metabolomics is an emerging technology that uses bioinformatics techniques and methods to comprehensively analyze changes in small-molecule metabolites within organisms to identify biomar-kers. In recent years, hair has gradually gained attention in the field of metabolomics. Compared with blood and urine, hair offers a wider detection window and enables retrospective studies. In addition, segmental analysis based on hair growth rates allows hair metabolomics to provide chronological information on changes in relevant substances. At present, hair metabolomics has established a certain research foundation in areas such as monitoring drug abuse, and it shows broad application prospects in forensic applications. This article reviews the collection and processing of hair samples, analytical techniques, and their applications in forensic research, with the aim of providing a reference for the further development of hair metabolomics and its in-depth application across multiple fields.

Key words: forensic medicine, metabolomics, hair, toxicological analysis, review

CLC Number:

Xin-ying WANG, Jia-li ZHANG, Ping XIANG, Xin WANG. Research Progress on Hair Metabolomics and Its Application in Forensic Science[J]. Journal of Forensic Medicine, 2026, 42(1): 49-55.

Figures/Tables 1

References 39

[1]	张高勤，王玫，张文芳，等. 滥用药物成瘾判定现状及代谢组学在其中的应用探讨[J].中国药物依赖性杂志，2011，20（1）：66-68.
	doi：0.13936/j.cnki.cjdd1992.2011.01.012.ZHANG G Q， WANG M， ZHANG W F， et al. Status of estimation of abused drugs addiction and its perspective based on metabonomics[J]. Zhongguo Yaowu Yilaixing Zazhi，2011，20（1）：66-68.
[2]	严慧，沈敏. 代谢组学在法医毒理学的应用进展[J].法医学杂志，2015，31（3）：219-226.
	doi：10.3969/j.issn.1004-5619.2015.03.012.YAN H， SHEN M. Development and application of metabonomics in forensic toxicology[J]. Fayixue Zazhi，2015，31（3）：219-226.
[3]	BAIS P， MOON-QUANBECK S M， NIKOLAU B J， et al. Plantmetabolomics.org： Mass spectro-metry-based arabidopsis metabolomics — Database and tools update[J]. Nucleic Acids Res，2012， 40（D1）：D1216-D1220. doi：10.1093/nar/gkr969 .
[4]	YANG Q J， ZHAO J R， HAO J， et al. Serum and urine metabolomics study reveals a distinct diagnostic model for cancer cachexia[J]. J Cachexia Sarcopenia Muscle，2018，9（1）：71-85. doi：10.1002/jcsm.12246 .
[5]	LINDON J C， HOLMES E， BOLLARD M E， et al. Metabonomics technologies and their applications in physiological monitoring， drug safety assessment and disease diagnosis[J]. Biomarkers，2004，9（1）：1-31. doi：10.1080/13547500410001668379 .
[6]	NAJAFOVA T， DAGDEVIREN G， KASIKCI M， et al. Segmental hair metabolomics analysis in pregnant women with pregnancy complications[J]. Metabolomics，2023，19（5）：45. doi：10.1007/s11306-023-02009-7 .
[7]	EISENBEISS L， STEUER A E， BINZ T M， et al. （Un）targeted hair metabolomics： First considera-tions and systematic evaluation on the impact of sample preparation[J]. Anal Bioanal Chem，2019，411（17）：3963-3977. doi：10.1007/s00216-019-01873-4 .
[8]	JANG W J， CHOI J Y， PARK B， et al. Hair metabolomics in animal studies and clinical settings[J]. Molecules，2019，24（12）：2195. doi：10.3390/mo-lecules24122195 .
[9]	MIN J Z， TOMIYASU Y， MOROTOMI T， et al. First observation of N-acetyl leucine and N-acetyl isoleucine in diabetic patient hair and quantitative analysis by UPLC-ESI-MS/MS[J]. Clin Chim Acta，2015，444：143-148. doi：10.1016/j.cca.2015.02.006 .
[10]	KEMPSON I M， LOMBI E. Hair analysis as a biomonitor for toxicology， disease and health status[J]. Chem Soc Rev，2011，40（7）：3915-3940. doi：10.1039/c1cs15021a .
[11]	JONES B， HAN T L， DELPLANCKE T， et al. Association between maternal exposure to phthalates and lower language ability in offspring derived from hair metabolome analysis[J]. Sci Rep，2018，8（1）：6745. doi：10.1038/s41598-018-24936-5 .
[12]	CHEN X Y， DE SEYMOUR J V， HAN T L， et al. Metabolomic biomarkers and novel dietary factors associated with gestational diabetes in China[J]. Metabolomics，2018，14（11）：149. doi：10.1007/s11306-018-1445-6 .
[13]	SULEK K， HAN T L， VILLAS-BOAS S G， et al. Hair metabolomics： Identification of fetal compromise provides proof of concept for biomarker discovery[J]. Theranostics，2014，4（9）：953-959. doi：10.7150/thno.9265 .
[14]	FAVRETTO D， COOPER G， ANDRAUS M， et al. The Society of Hair Testing consensus on gene-ral recommendations for hair testing and drugs of abuse testing in hair[J]. Drug Test Anal，2023，15（9）：1042-1046. doi：10.1002/dta.3526 .
[15]	KINTZ P， CIRIMELE V， JAMEY C， et al. Tes-ting for GHB in hair by GC/MS/MS after a single exposure. application to document sexual assault[J]. J Forensic Sci，2003，48（1）：195-200. doi：10.1520/JFS2002209 .
[16]	CHOI B， KIM S P， HWANG S， et al. Metabolic characterization in urine and hair from a rat model of methamphetamine self-administration using LC-QTOF-MS-based metabolomics[J]. Metabolomics，2017，13（10）：119. doi：10.1007/s11306-017-1257-0 .
[17]	CHANG W C， WANG P H， CHANG C W， et al. Extraction strategies for tackling complete hair metabolome using LC-HRMS-based analysis[J]. Talanta，2021，223（Pt 1）：121708. doi：10.1016/j.talanta.2020.121708 .
[18]	XIE P， WANG T J， YIN G， et al. Metabonomic study of biochemical changes in human hair of heroin abusers by liquid chromatography coupled with ion trap-time of flight mass spectrometry[J]. J Mol Neurosci，2016，58（1）：93-101. doi：10.1007/s12031-015-0655-x .
[19]	JOO K M， KIM A R， KIM S N， et al. Metabolomic analysis of amino acids and lipids in human hair altered by dyeing， perming and bleaching[J]. Exp Dermatol，2016，25（9）：729-731. doi：10.1111/exd.13025 .
[20]	杜航，龚进，代华，等. 慢性砷中毒患者头发代谢物特征的代谢组学研究[J].环境与职业医学，2018，35（2）：163-167. doi：10.13213/j.cnki.jeom.2018.17472 .
	DU H， GONG J， DAI H， et al. Metabonomics study on metabolic profile of hair samples from chronic arsenic poisoning patients[J]. Huanjing Yu Zhiye Yixue，2018，35（2）：163-167.
[21]	KIM S， JANG W J， YU H， et al. Revealing metabolic perturbation following heavy methamphe-tamine abuse by human hair metabolomics and network analysis[J]. Int J Mol Sci，2020，21（17）：6041. doi：10.3390/ijms21176041 .
[22]	The Metabolomics Innovation Centre. Human Metabolome Database[DB/OL]. （2005-01-25） [2023-12-25]. .
[23]	ALONSO A， MARSAL S， JULIÀ A. Analytical methods in untargeted metabolomics： State of the art in 2015[J]. Front Bioeng Biotechnol，2015，3：23. doi：10.3389/fbioe.2015.00023 .
[24]	SON H H， LEE D Y， SEO H S， et al. Hair sterol signatures coupled to multivariate data analysis reveal an increased 7β-hydroxycholesterol production in cognitive impairment[J]. J Steroid Biochem Mol Biol，2016，155（Pt A）：9-17. doi：10.1016/j.jsbmb.2015.09.024 .
[25]	JUNG H J， KIM S J， LEE W Y， et al. Gas chromatography/mass spectrometry based hair steroid profiling may reveal pathogenesis in hair follicles of the scalp[J]. Rapid Commun Mass Spectrom，2011，25（9）：1184-1192. doi：10.1002/rcm.4975 .
[26]	BOUHIFD M， HARTUNG T， HOGBERG H T， et al. Review： Toxicometabolomics[J]. J Appl Toxicol，2013，33（12）：1365-1383. doi：10.1002/jat.2874 .
[27]	SZEREMETA M， PIETROWSKA K， NIEMCUNO-WICZ-JANICA A， et al. Applications of metabolomics in forensic toxicology and forensic medicine[J]. Int J Mol Sci，2021，22（6）：3010. doi：10.3390/ijms22 063010 .
[28]	谢普. 吸毒者头发中毒品的分析和其代谢组学研究[D].沈阳：沈阳药科大学，2015.
	XIE P. Analysis of illict drugs and metabolic research in hair of drug abusers[D]. Shenyang： Shenyang Pharmaceutical university， 2015.
[29]	SEO M J， SONG S H， KIM S， et al. Mass spectrometry-based metabolomics in hair from current and former patients with methamphetamine use disorder[J]. Arch Pharmacal Res，2021，44（9/10）：890-901. doi：10.1007/s12272-021-01353-3 .
[30]	ERB S. Evaluation of the relationship between anxiety during withdrawal and stress-induced reinstatement of cocaine seeking[J]. Prog Neuro Psychopharmacol Biol Psychiatry，2010，34（5）：798-807. doi：10.1016/j.pnpbp.2009.11.025 .
[31]	PARROTT A C， SANDS H R， JONES L， et al. Increased cortisol levels in hair of recent Ecstasy/MDMA users[J]. Eur Neuropsychopharmacol，2014，24（3）：369-374. doi：10.1016/j.euroneuro.2013.11.006 .
[32]	VOEGEL C D， KROLL S L， SCHMID M W， et al. Alterations of stress-related glucocorticoids and endocannabinoids in hair of chronic cocaine users[J]. Int J Neuropsychopharmacol，2022，25（3）：226-237. doi：10.1093/ijnp/pyab070 .
[33]	YANG J， LI J F， XU G Y， et al. Elevated hair cortisol levels among heroin addicts on current methadone maintenance compared to controls[J]. PLoS One，2016，11（3）：e0150729. doi：10.1371/journal.pone.0150729 .
[34]	GROSVENOR A J， DEB-CHOUDHURY S， MIDDLEWOOD P G， et al. The physical and chemical disruption of human hair after bleaching — Studies by transmission electron microscopy and redox proteomics[J]. Int J Cosmet Sci，2018，40（6）：536-548. doi：10.1111/ics.12495 .
[35]	PETZEL-WITT S， MEIER S I， SCHUBERT-ZSILAVECZ M， et al. PTCA （1H-pyrrole-2， 3， 5-tricarboxylic acid） as a marker for oxidative hair treatment[J]. Drug Test Anal，2018，10（4）：768-773. doi：10.1002/dta.2305 .
[36]	EISENBEISS L， BINZ T M， BAUMGARTNER M R， et al. A possible new oxidation marker for hair adulteration： Detection of PTeCA （1H-pyrrole-2，3，4，5-tetracarboxylic acid） in bleached hair[J]. Drug Test Anal，2020，12（2）：230-238. doi：10.1002/dta.2713 .
[37]	EISENBEISS L， BINZ T M， BAUMGARTNER M R， et al. Cheating on forensic hair testing? Detection of potential biomarkers for cosmetically altered hair samples using untargeted hair metabolomics[J]. Analyst，2020，145（20）：6586-6599. doi：10.1039/d0an01265c .
[38]	HU J， LIU W， ZHANG S J， et al. Retrospective analysis of laboratory data from 19，275 hair samples submitted for drug abuse/misuse analysis from February 2019 to June 2021[J]. Forensic Sci Int，2022，337：111350. doi：10.1016/j.forsciint.2022. 111350 .
[39]	THORSPECKEN J， SKOPP G， PÖTSCH L. In vitro contamination of hair by marijuana smoke[J]. Clin Chem，2004，50（3）：596-602. doi：10.1373/clinchem.2003.026120 .