Abstract: Objective To develop a specific, sensitive, reproducible SPE-HPLC method for the determination of 37 drugs in whole blood. Methods With the doxapram as internal standard, Oasis column was used to extract drugs from whole blood. Two kinds of mobile phases were used in this study. Separations were achieved by a LiChrospher?誖 100 RP-C18 (250 mm×4.0 mm×5 μm)column kept at 50 ℃, the DAD detector was set at 230 nm and 250 nm. Results The limit of detection were 1～30 ng/mL. The method showed excellent linearity and the linear correlation coefficient was≥0.997 98. The relative standard deviation for between-day and within-day assay were <10%. Conclusion The method is effective, simple, reliable and has been used in real cases.

Key words: SPE, HPLC, whole blood