Abstract: Objective To explore the method for DNA typing in formalin-fixed tissue by detecting SNP markers on X chromosome. Methods Genomic DNA was prepared from formalin-fixed tissue. In the event that typing using SinofilerTM and MiniFilerTM kits had failed, 51 SNPs were amplified with multiplex PCR and typed with matrix-assisted laser desorption/ionization-time of flight mass spectrometry（MALDI-TOF MS）. Results Full profiles of X-SNP loci were obtained from the formalin-fixed tissue, while the STR and miniSTR genotyping failed. Conclusion SNP genotyping technique can be used to obtain more information for formalin fixed tissues.

Key words: forensic genetics, polymorphism, single nucleotide, formaldehyde, individual identification