Abstract: Objective To explore the tissue-specific gene expressions of the peripheral blood and the menstrual blood, and to search some specific factors to establish an effective method for identifying the peripheral blood and the menstrual blood. Methods The specific products of the peripheral blood and the menstrual blood were detected by RT-PCR and separated by electrophoretic technology. Results Beta-spectrin （SPTB） as one specific marker of peripheral blood and 18S rRNA as a kind of the housekeeping gene were expressed in both the peripheral blood and the menstrual blood. However, matrix metalloproteinase 7 （MMP7） as one specific marker of menstrual blood and human beta defensin 1 （HBD1） as one specific marker of vaginal discharge were only found in the menstrual blood. Conclusion There are differences of specific gene expressions between the peripheral blood and the menstrual blood. They could be accurately distinguished from each other by using the combination of fluorescence technology and RT-PCR to detect the specific identification of mRNA.

Key words: forensic genetics, menstrual blood, peripheral blood, identification