Abstract: Objective To identify the Y-chromosomal genetic types for the soldier’s remains from Huaihai Campaign, and to offer a clue for search of their paternal relatives. Methods DNA of the remains were extracted by the ancient DNA extraction method. Yfiler kit was used for the multiplex amplification of 17 Y-STR loci. The haplogroups of the samples were speculated. Detailed genotyping of the selected Y-SNP was performed based on the latest Y-chromosome phylogenetic tree. Haplotype-sharing analysis was done based on the data of Y-SNP and Y-STR, the closest modern individual information to the genetic relationship of remains was gained. Results A total of 8 Y-STR haplotypes were observed on 17 Y-STR loci of 8 male individuals. Furthermore, 6 Y-SNP haplogroups were identified, which were O2a1-M95+, O1a1-P203+, O3*-M122+/M234-, D1-M15+, C3*-ST and R1a1-M17+. Conclusion Identification of Y-chromosomal genetic types for the soldier’s remains from Huaihai Campaign shows a reference value on inferring the geographical origins of old materials.

Key words: forensic genetics, Y chromosome, military personnel, personal identification, Huaihai Campaign