Journal of Forensic Medicine ›› 2022, Vol. 38 ›› Issue (4): 468-472.DOI: 10.12116/j.issn.1004-5619.2020.400907
• Original Article • Previous Articles Next Articles
Yan-ru XI(), Yuan-xin LIU, Na FENG, Zhen GU, Jun-hong SUN, Jie CAO, Qian-qian JIN, Qiu-xiang DU(
)
Received:
2020-09-15
Online:
2022-08-25
Published:
2022-08-28
Contact:
Qiu-xiang DU
CLC Number:
Yan-ru XI, Yuan-xin LIU, Na FENG, Zhen GU, Jun-hong SUN, Jie CAO, Qian-qian JIN, Qiu-xiang DU. Application of Linear Regression Model of Gpnmb Gene in Rat Injury Time Estimation[J]. Journal of Forensic Medicine, 2022, 38(4): 468-472.
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URL: http://www.fyxzz.cn/EN/10.12116/j.issn.1004-5619.2020.400907
基因 | 基因ID1) | 引物和探针序列(5′→3′) | 产物长度/bp |
---|---|---|---|
Rpl13 | NM_031101.1 | 上游:TCGTGAGGTGCCCTACAGTTAG; 下游:GGTGCGTGCCATTTTCTTGTG; 探针:CACACCAAGGTCCGGGCTGGCAG | 107 |
Rpl32 | NM_013226.2 | 上游:ATCTGGCCCTTGAATCTTCTCC; 下游:AGAGGACCAAGAAGTTCATCAGG; 探针:TGTCGATGCCTCTGGGTTTCCGCC | 115 |
Gpnmb | NM_133298.1 | 上游:CATCGTCTGCCCCTGTGGTCCAGT; 下游:GGCAATATCGTCTATGAGAGGAAC; 探针:TGGTGTTGTCTTCCCAGTCC | 91 |
Tab. 1 Sequences of primers and probes
基因 | 基因ID1) | 引物和探针序列(5′→3′) | 产物长度/bp |
---|---|---|---|
Rpl13 | NM_031101.1 | 上游:TCGTGAGGTGCCCTACAGTTAG; 下游:GGTGCGTGCCATTTTCTTGTG; 探针:CACACCAAGGTCCGGGCTGGCAG | 107 |
Rpl32 | NM_013226.2 | 上游:ATCTGGCCCTTGAATCTTCTCC; 下游:AGAGGACCAAGAAGTTCATCAGG; 探针:TGTCGATGCCTCTGGGTTTCCGCC | 115 |
Gpnmb | NM_133298.1 | 上游:CATCGTCTGCCCCTGTGGTCCAGT; 下游:GGCAATATCGTCTATGAGAGGAAC; 探针:TGGTGTTGTCTTCCCAGTCC | 91 |
组别 | 死后0 h | 0 ℃ | 16 ℃ | 26 ℃ | |||
---|---|---|---|---|---|---|---|
死后12 h | 死后24 h | 死后12 h | 死后24 h | 死后12 h | 死后24 h | ||
损伤后0 h | 1.00±0.25 | 0.76±0.20 | 1.50±0.31 | 2.20±0.50 | 1.52±0.15 | 1.87±0.35 | 0.72±0.15 |
损伤后4 h | 1.97±0.46 | 2.76±0.68 | 1.58±0.18 | 2.21±0.36 | 1.79±0.42 | 2.31±0.41 | 7.02±2.08 |
损伤后8 h | 2.53±0.50 | 3.51±0.83 | 4.33±1.16 | 4.00±0.99 | 3.64±1.31 | 5.10±0.97 | 9.84±2.50 |
损伤后12 h | 9.20±1.23 | 13.87±4.51 | 16.75±3.32 | 7.98±1.83 | 12.52±2.45 | 5.52±1.69 | 12.49±3.47 |
损伤后16 h | 13.71±4.14 | 16.57±5.46 | 13.38±1.80 | 18.33±5.13 | 15.56±7.90 | 21.37±8.30 | 16.64±1.88 |
损伤后20 h | 15.48±6.27 | 19.75±2.31 | 25.71±9.46 | 23.23±5.03 | 16.91±6.89 | 23.27±9.53 | 29.57±11.61 |
损伤后24 h | 30.18±10.82 | 21.52±2.89 | 28.41±7.07 | 33.54±2.31 | 13.45±3.37 | 32.02±3.16 | 33.49±4.46 |
Tab. 2 The expression of Gpnmb mRNA varied with the injury time at different time of death and postmortem storage temperature
组别 | 死后0 h | 0 ℃ | 16 ℃ | 26 ℃ | |||
---|---|---|---|---|---|---|---|
死后12 h | 死后24 h | 死后12 h | 死后24 h | 死后12 h | 死后24 h | ||
损伤后0 h | 1.00±0.25 | 0.76±0.20 | 1.50±0.31 | 2.20±0.50 | 1.52±0.15 | 1.87±0.35 | 0.72±0.15 |
损伤后4 h | 1.97±0.46 | 2.76±0.68 | 1.58±0.18 | 2.21±0.36 | 1.79±0.42 | 2.31±0.41 | 7.02±2.08 |
损伤后8 h | 2.53±0.50 | 3.51±0.83 | 4.33±1.16 | 4.00±0.99 | 3.64±1.31 | 5.10±0.97 | 9.84±2.50 |
损伤后12 h | 9.20±1.23 | 13.87±4.51 | 16.75±3.32 | 7.98±1.83 | 12.52±2.45 | 5.52±1.69 | 12.49±3.47 |
损伤后16 h | 13.71±4.14 | 16.57±5.46 | 13.38±1.80 | 18.33±5.13 | 15.56±7.90 | 21.37±8.30 | 16.64±1.88 |
损伤后20 h | 15.48±6.27 | 19.75±2.31 | 25.71±9.46 | 23.23±5.03 | 16.91±6.89 | 23.27±9.53 | 29.57±11.61 |
损伤后24 h | 30.18±10.82 | 21.52±2.89 | 28.41±7.07 | 33.54±2.31 | 13.45±3.37 | 32.02±3.16 | 33.49±4.46 |
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