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28 June 1986, Volume 2 Issue 3 Previous Issue    Next Issue
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Studies on the Genetic Polymorphism of the Red Cell GPT in Chinese and Determination of the GPT Types from Blood Stains CHEN YUELONG AND GUO JINGYUAN DEPARTMENT OF FORENSIC SEROLOGY SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES 1986, 0(3): 1-7.  Abstract ( 1873 )   Summary Genetic polymorphism of soluble Glutamic-Pyruvic Transaminase (GPT) was first described by Chen et al in 1971. Three common phenotypes were found. The three phenotypes are controlled by a pair of alleles Gpt~1and Gpt~2 at an autosomal locus. The polymorphism of GPT have been studied in different population groups and so far little information is available for China. This paper reported the polymorphism of GPT examined in 277 normal unrelated donors living in Guangzhou. Enzyme typing was carried out for starch-gel and polyacrlamide-gel electrophoresis and the specific enzyme staining. The gene frequencies of Gpt~1 and Gpt~2 were estimated at 0.525 and 0.475. The observed numbers of the phenotypes were in good agreement with the numbers expected under the Hardy-weinberg equilibrium (x~2=1.802,0.2>p>0.1). The paternity exclusion rate and DP (Discriminating Power) were estimated at 0.187 and 0.642. The activity of the three phenotypes were measured in the red cell lysates. The mean activity of GPT phenotypes were 4.433±0.643 EU/gHb for GPTl,2.900±0.835 EU/gHb for GPT2-1 and 2.433, ±0.870 EU/gHq for GPT2. The GPT phenotypes of bloob stains adhered to gauze, filter paper, white cotton cloth and glass plate can be detected at 4℃ for two weeks, 52?? for four days and 37℃ for one day. GPT1 has longer examinable time than GPT2-1 and G PT2. It may be seen that the examination of GPT phenotypes is one of the available technique for determination of paternity and identification. Related Articles | Metrics

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Gm and Km Grouping of Dried Bloodstains ZHU YONGMING ZHAO TONGMAO (SHANGHAI INSTITUTE OF BLOOD TRANSFUSION) 1986, 0(3): 7-10.  Abstract ( 1900 )   This paper introduces a simple method of Gm and Km grouping of dried bloodstains. The detection of Gm (1), (2), (3), (5), (21) and Km (1) factors in bloodstains aged for 1 year at room temperature has been observed. The correlation between the weight of dried blood and the volume of fresh blood was set up. Related Articles | Metrics

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Distribution of GLOI Phenotypes in Wuhan Population YU CHUNYING LONG YAN YANG RONGZHI YANG QINGEN DEPT OF FORENSIC MEDICINE;TONG JI MED CAL UNIVERSITY 1986, 0(3): 11-12.  Abstract ( 1901 )   The distribution of GLOI Phenotype was examined by mixed starch/agarose gel from 211 healthy donor in Wuhan. The phenotype freqencies in Wuhan were as follows: GLOI1-1=7(3.32%)、GLOI 2-1=52 (24.64%), GLOI 2-2=152(72.04%).The gene freqencies calculated were at GLOI~1 = 0.1564、GLOI~2=0.8436. The discriminating power of GLOI was 0.4192. Related Articles | Metrics

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THE PRELININARY USAGE OF ANTI-A MONOLONAL ANTIBODY ZHANG JIAN;ZHANG XIU-MEI;FU BEI-CHUAN;GUO YANG;DUAN WEI 1986, 0(3): 13-16.  Abstract ( 1933 )   A monoclonal anti-A antibody has been used to test human blood group A Substance Antigen Monoclonal Anti-A has higher specificty when compared with anti-A sera of human origin. It is suitable reagent for testing A Substance antigen of blood stains. Related Articles | Metrics

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The Use Valne in Forensic Identification of Blood Cholinesterase as an Index Deduce the Death of DDVP poison YAN LING;BU JUN;DENG LIPING;ZHU GUANGYOU 1986, 0(3): 16-20.  Abstract ( 2140 )   There are two indices-lethal oral dose and actinity values of blood cholinesterase-to identificaie death of organic phosphorous poison in forensic medicine. But both of them have not yet got a celarcut idea or definition, so that there are difficulties in the work of identification. In order to estimate the value of them, these two indices were compared, and a the acetyl cholinesterase (AChE) is the target organ's substance, it was supposed AChE would be a more sensitive index. In the experiment, 80 female sprague-Dawley rates were divided into 4 groups with the oral dose of 38. 7, 44. 4, 51. 1 and 58. 7 mg/kg DDVP respectively, and their fatalty rate and blood ChE activity were recorded. The results were theat there was no significant difference of ChE activity values between doses but high significant difference ( P<0.01= between survival and death. It is indicated that it does have a lethal dose of ChE, which can be used into forensic identification. And after the indices compared, it is showed that the ChE are much more sensitive than the oral dose of DDVP, that is to say 3.9 times the efficiency of DDVP oral dose. The methods of estimation-the definite value and the suspect zone--have been discussed. Related Articles | Metrics

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Observation of 54 Species of Animal Hair with SEM ZHU XIAO-MAN;GUO YUN-RONG;XIAO JIAN-ZHONG;CHEN YING (DEPARTMENT OF CLINICAL FORENSIC MEDICINE;SUN YAT-SEN UNIVERSITY OF MEDICAL SCIENCES 1986, 0(3): 20-27.  Abstract ( 2051 )   The authors report the features of cuticle scales which were observed in 54 species of animal hair under SEM ( Scanning Electron Microscope ). According to the margin of morphological characteristics, cuticle scales can be classified to three types. Serrated type, Smooth type, Mixture type. Besides, it is also showed that some cuticle scales are lost partly or completely. Related Articles | Metrics