法医学杂志

法医学杂志 ›› 2018, Vol. 34 ›› Issue (5): 492-494.DOI: 10.12116/j.issn.1004-5619.2018.05.007

所属专题： 死亡时间推断

• 专题 • 上一篇    下一篇

大鼠死后肝、脾、肾电导率与早期死亡时间的关系

郑  哲，翟仙敦，夏志远，赵琳琳，李永林，莫耀南   

  1. 河南科技大学法医学院，河南 洛阳 471003
  • 发布日期:2018-10-25 出版日期:2018-10-28
  • 通讯作者: 莫耀南，男，教授，硕士研究生导师，主要从事法医病理学和法医临床学研究；E-mail：forensic@haust.edu.cn
  • 作者简介:郑哲（1991—），男，硕士研究生，主要从事法医病理学研究；E-mail：417071857@qq.com

Relationship between Early Postmortem Interval and Electrical Conductivity of Rat Liver, Spleen and Kidney after Death in Rats

ZHENG Zhe, ZHAI Xian-dun, XIA Zhi-yuan, ZHAO Lin-lin, LI Yong-lin, MO Yao-nan   

  1. School of Forensic Medicine, Henan University of Science and Technology, Luoyang 471003, Henan Province, China
  • Online:2018-10-25 Published:2018-10-28

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摘要： 目的 测定24 h内不同死亡时间（postmortem interval，PMI）大鼠肝、脾、肾的电导率（electrical conductivity，EC），研究不同器官EC与早期PMI的关系。 方法 将45只SD大鼠采用颈椎脱臼的方法处死后保存于25 ℃的环境中，分别于死后0、3、6、9、12、15、18、21、24 h取大鼠肝、脾、肾组织，用去离子水制成质量浓度为0.1 g/mL的浸渍液，测定不同器官浸渍液的EC值，分别建立不同器官EC值与PMI的回归方程，分析大鼠死后不同器官组织EC值与早期PMI的关系。 结果 大鼠死后24 h内，肝、脾组织EC值与PMI的关系用一次方程拟合较好，其中以肝组织最好，脾组织次之，而肾组织的EC值在24 h内变化规律不显著。肝、脾EC与早期PMI呈现出良好的线性关系。 结论 大鼠死后，肝、脾两种组织的EC值与PMI相关性较好，均可用于早期PMI推断。

关键词: 法医病理学, 死亡时间, 电导率, 肝, 脾, 肾, 大鼠

Abstract: Objective To determine the electrical conductivity （EC） of the liver, spleen and kidney of rats at different postmortem intervals （PMIs） within 24 hours for investigating the relationship between EC of different organs and early PMI. Methods Totally 45 SD rats were sacrificed by cervical dislocation and kept at a constant temperature of 25 ℃. Tissues were taken from the liver, spleen, and kidney of rats at 0, 3, 6, 9, 12, 15, 18, 21 and 24 h. Impregnating solution with a mass concentration 0.1 g/mL was prepared using deionized water. The EC value of impregnating solution with different organs was separately determined. The regression equations of EC and PMI for different organs were established, respectively. The relationship between EC of different organs and early PMI was analysed in deceased rats. Results The relationship between PMI and EC of the liver and spleen was well fitted with the linear equation. The liver showed the best fitting degree followed by the spleen, while the EC of the kidney showed no significant changes within 24 h. There was a good linear relationship between early PMI and the EC of the liver and spleen. Conclusion A good linear relationship between early PMI and the EC of the liver and spleen can be found in rats after death, which can be used for the early PMI estimation.

Key words: forensic pathology, postmortem interval, electrical conductivity, liver, spleen, kidney, rats