法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (4): 396-401.DOI: 10.12116/j.issn.1004-5619.2019.04.003

• 论著 • 上一篇    下一篇

过敏性休克死亡豚鼠肺组织中CD63的表达

张楚楠， 于浩， 王晓龙， 王鹏飞， 冯春梅， 尤家斌， 王昌亮， 徐国辉， 张国华   

  1. 中国医科大学法医学院，辽宁 沈阳 110122）
  • 发布日期:2019-08-25 出版日期:2019-08-28
  • 通讯作者: 张国华，男，教授，主要从事法医学教学、科研和鉴定；E-mail：ghzhang@cmu.edu.cn
  • 作者简介:张楚楠（1992—），男，硕士，主要从事法医病理学鉴定和研究；E-mail：1154874846@qq.com
  • 基金资助:
    辽宁省自然科学基金资助项目（2015020511）

Expression of CD63 in Lung Tissue of Guinea Pigs Dying of Anaphylactic Shock

ZHANG Chu-nan, YU Hao, WANG Xiao-long, WANG Peng-fei, FENG Chun-mei, YOU Jia-bin, WANG Chang-liang, XU Guo-hui, ZHANG Guo-hua   

  1. School of Forensic Medicine, China Medical University, Shenyang 110122, China
  • Online:2019-08-25 Published:2019-08-28

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摘要： 目的 研究过敏性休克死亡豚鼠肺组织中分化抗原簇63（cluster of differentiation 63，CD63）的蛋白表达，探讨CD63对过敏性休克死亡的诊断价值。 方法 20只豚鼠随机分为对照组，过敏性休克死亡即刻组、冷藏组（4 ℃贮藏48 h）、冷冻组（-20 ℃贮藏7 d）。采用人混合血清注射建立过敏性休克死亡豚鼠动物模型。应用苏木精-伊红（hematoxylin-eosin，HE）染色、免疫组织化学染色、Western印迹法、酶联免疫吸附测定（enzyme-linked immunosorbent assay，ELISA）和real-time RT-PCR检测肺组织中CD63蛋白及CD63 mRNA的表达变化。 结果 HE染色结果显示，各过敏性休克组肺组织淤血、水肿，嗜酸性粒细胞浸润。Western印迹法检测结果显示，过敏性休克豚鼠肺组织中CD63蛋白表达高于对照组（P<0.05），各过敏性休克组组间比较，差异无统计学意义。免疫组织化学染色、real-time RT-PCR结果与Western印迹法结果一致。ELISA检测结果显示，死亡即刻组CD63蛋白表达高于对照组（P<0.05）。 结论 过敏性休克死亡豚鼠肺组织中CD63蛋白和CD63 mRNA表达明显增强。动物尸体冷藏48 h和冷冻7 d不影响上述指标的检查，CD63有望成为过敏性休克死亡的辅助诊断指标。

关键词: 法医病理学, 过敏反应, 休克, 肺, 分化抗原簇63, 豚鼠

Abstract: Objective To study the protein expression of cluster of differentiation 63 （CD63） in lung tissues of guinea pigs that died of anaphylactic shock and discuss the diagnostic value of CD63 for death from anaphylactic shock. Methods Twenty guinea pigs were randomly divided into control group, anaphylactic shock immediate death group, cold storage group （4 ℃ for 48 h） and frozen group （-20 ℃ for 7 d）. The animal model of guinea pigs that died of anaphylactic shock was established with human mixed serum injection. The expression changes of CD63 protein and CD63 mRNA in lung tissues were detected by hematoxylin-eosin （HE） staining, immunohistochemical staining, Western blotting, enzyme-linked immunosorbent assay （ELISA） and real-time RT-PCR. Results HE staining results showed congestion, and edema of lung tissues, and eosinophil infiltration in the anaphylactic shock groups. Western blotting analysis results showed that the expression of CD63 protein in the lung tissues of guinea pigs that died of anaphylactic shock was significantly higher than that in the control group （P<0.05）. Comparison between the anaphylactic shock groups was made, and the differences had no statistical significance. The results of immunohistochemical staining and real-time RT-PCR were consistent with that of Western blotting. ELISA results showed that CD63 protein expression in the immediate death group was higher than that in the control group （P<0.05）. Conclusion The expression of CD63 protein and CD63 mRNA in the lung tissues of guinea pigs that died of anaphylactic shock is significantly enhanced. Animal carcasses which were put in cold storage for 48 h and frozen for 7 d do not affect the examination of the above indicators. CD63 protein is expected to become an auxiliary diagnostic indicator of death from anaphylactic shock.

Key words: forensic pathology, anaphylaxis, shock, lung, cluster of differentiation 63 （CD63）, guinea pig