法医学杂志

法医学杂志 ›› 2019, Vol. 35 ›› Issue (5): 572-575.DOI: 10.12116/j.issn.1004-5619.2019.05.011

• 论著 • 上一篇    下一篇

大鼠骨骼肌浸渍液电导率及其化学物质含量与死亡时间的关系

郑哲1， 翟仙敦1， 夏志远2， 李永林1， 赵琳琳1， 刘慧丽3， 莫耀南1   

  1. 1. 河南科技大学法医学院，河南 洛阳 471003； 2. 中国政法大学证据科学研究院，北京 100088； 3. 河南科技大学医院，河南 洛阳 471003）
  • 发布日期:2019-10-25 出版日期:2019-10-28
  • 通讯作者: 莫耀南，男，教授，硕士研究生导师，主要从事法医病理学和法医临床学研究；E-maill：forensic@haust.edu.cn
  • 作者简介:郑哲（1991—），男，硕士研究生，主要从事法医病理学研究；E-mail：forensiczheng@sina.com
  • 基金资助:
    河南省基础与前沿课题资助项目（112300410082）

Relationship between Electrical Conductivity and Chemical Content of Rat Skeletal Muscle Impregnating Solution and Postmortem Interval

ZHENG Zhe1, ZHAI Xian-dun1, XIA Zhi-yuan2, LI Yong-lin1, ZHAO Lin-lin1, LIU Hui-li3, MO Yao-nan1   

  1. 1. School of Forensic Medicine, Henan University of Science and Technology, Luoyang 471003, Henan Province, China; 2. Institute of Evidence Law and Forensic Science, China University of Political Science and Law, Beijing 100088, China; 3 Hospital of Henan University of Science and Technology, Luoyang 471003, Henan Province, China
  • Online:2019-10-25 Published:2019-10-28

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摘要： 目的 研究骨骼肌组织浸渍液电导率中化学离子随死亡时间（postmortem interval，PMI）的变化机制。 方法 将健康SD大鼠处死后保存于25 ℃左右的环境中，于死后即刻（0 d）、1 d、2 d、3 d、4 d、5 d、6 d和7 d提取大鼠骨骼肌组织，并与去离子水混合制成质量浓度为0.1 g/mL的浸渍液，测定骨骼肌组织浸渍液EC值及常见的钾离子、钠离子、氯离子等9种化学成分的含量。 结果 大鼠死后7 d内骨骼肌EC随PMI的延长而逐渐增大（P=0.024），其浸渍液中尿酸（P=0.032）、尿素氮（P=0.013）、无机磷（P=0.022）的含量随PMI的延长而逐渐升高，镁离子的含量则随PMI的延长而逐渐降低（P=0.047），而钾离子、钠离子、氯离子、钙离子和肌酐与PMI相关性较弱（P>0.05）。 结论 大鼠死后骨骼肌浸渍液中尿酸、尿素氮、无机磷等化学成分含量变化是骨骼肌EC变化的分子基础，有望通过多指标联用提高EC推断PMI的准确性。

关键词: 法医病理学, 骨骼肌, 电导率, 死亡时间推断, 大鼠

Abstract: Objective To study the mechanism of change of the electrical conductivity （EC） of rat skeletal muscle impregnating solution that occurs with the change of postmortem interval （PMI）. Methods Healthy Sprague-Dawley rats were killed and kept at about 25 ℃. Skeletal muscles were extracted at different PMI——immediate （0 d）, 1 d, 2 d, 3 d, 4 d, 5 d, 6 d, and 7 d, then mixed with deionized water to make impregnating solution with a mass concentration of 0.1 g/mL. The solution’s EC and nine common chemicals in it, such as potassium ion, calcium ion, and chloride ion, were determined. Results EC increased gradually with the extending of PMI （P=0.024） during the 7 days after the rats’ death. The content of uric acid （P=0.032）, urea nitrogen （P=0.013） and phosphorus （P=0.022） also increased during the extension. However, the content of magnesium ions decreased with extending of PMI （P=0.047）. The correlation between potassium ion, sodium ion, chlorine ion, calcium ion, creatinine and PMI were weak （P>0.05）. Conclusion The molecular basis of skeletal muscle EC change in rats after their death is the changes of uric acid, urea nitrogen, inorganic phosphorus and other chemical components. Furthermore, combine use of various indicators can improve the accuracy of the EC method to infer PMI.

Key words: forensic pathology, skeletal muscle, electrical conductivity, estimation of postmortem interval, rats