法医学杂志

法医学杂志 ›› 2021, Vol. 37 ›› Issue (2): 211-214.DOI: 10.12116/j.issn.1004-5619.2019.490414

• 技术与应用 • 上一篇    下一篇

过敏者外周血DNA甲基化差异

刘明哲1， 郭华林1， 冯彦2， 李建国3， 李鹏飞1， 高彩荣1， 郭相杰1   

  1. 1. 山西医科大学法医学院，山西 晋中 030600； 2. 山西医科大学第一医院，山西 太原 030000； 3. 中国辐射防护研究院，山西 太原 030000
  • 收稿日期:2019-04-25 发布日期:2021-04-25 出版日期:2021-04-28
  • 通讯作者: 郭相杰，男，副教授，硕士研究生导师，主要从事法医病理学研究；E-mail：258187101@qq.com
  • 作者简介:刘明哲（1992—），男，硕士，主要从事过敏性休克死亡相关法医学研究；E-mail：1435884000@qq.com
  • 基金资助:
    国家自然科学基金面上项目（81971790）；山西省科技厅自然科学基金面上项目（201601D102070）；山西医科大学博士启动基金资助项目（03201536）

DNA Methylation Differences in Peripheral Blood of Patients with Anaphylaxis

LIU Ming-zhe1, GUO Hua-lin1, FENG Yan2, LI Jian-guo3, LI Peng-fei1, GAO Cai-rong1, GUO Xiang-jie1#br#   

  1. 1. School of Forensic Medicine, Shanxi Medical University, Jinzhong 030600, Shanxi Province, China; 2. First Hospital of Shanxi Medical University, Taiyuan 030000, China; 3. China Institute for Radiation Protection, Taiyuan 030000, China
  • Received:2019-04-25 Online:2021-04-25 Published:2021-04-28

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摘要： 目的 通过研究头孢类药物过敏性休克死亡者外周血有核细胞DNA甲基化情况，为药物过敏性休克死亡的法医学诊断提供新的研究方向和依据。 方法 运用甲基化芯片检测头孢类药物过敏性休克死亡者和免疫正常者外周血有核细胞DNA甲基化情况。使用R语言methylkit、ChAMP包分别对测序数据、芯片数据进行DNA甲基化差异分析，运用随机森林算法对所得DNA甲基化差异位点重要性进行评估。 结果 在ETS1、PRR23B、GNAS等基因座上获得与头孢类药物过敏高度相关的DNA甲基化差异位点。 结论 头孢类药物过敏与DNA甲基化相关，DNA甲基化有望成为过敏性休克死亡的法医学鉴定新策略。

关键词: 法医病理学, 过敏反应, DNA甲基化, DNA芯片, 药物过敏

Abstract: Objective To study the DNA methylation of nucleated cells in peripheral blood of patients died from anaphylactic shock caused by cephalosporin drugs and to provide a new research direction and basis for the forensic diagnosis of shock caused by drug hypersensitiveness. Methods Methylation microarray was used to detect DNA methylation of nucleated cells in peripheral blood of patients died from anaphylactic shock caused by cephalosporin drugs and normal subjects. Sequencing data and chip data were analyzed for differences in DNA methylation using R language methylkit, ChAMP package. Random forest algorithm was used to evaluate the importance of the DNA methylation differential sites. Results Differential sites of DNA methylation highly associated with anaphylaxis caused by cephalosporin drugs were obtained at loci such as ETS1, PRR23B and GNAS. Conclusion Cephalosporin allergy is associated with DNA methylation, and DNA methylation may be a new strategy for forensic identification of anaphylactic shock and death.

Key words: forensic pathology, anaphylactic response, DNA methylation, DNA chip, drug anaphylaxis

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