法医学杂志

法医学杂志 ›› 2015, Vol. 31 ›› Issue (5): 340-342.DOI: 10.3969/j.issn.1004-5619.2015.05.002

• 论著 • 上一篇    下一篇

过敏性猝死豚鼠不及时冷藏对血清IgE的影响及法医学意义

苏  俊1，余  舰2，刘  勇2   

  1. （1. 遵义医学院附属医院病理科，贵州 遵义 563003； 2. 遵义医学院附属医院司法鉴定中心，贵州 遵义 563003）
  • 发布日期:2015-10-25 出版日期:2015-10-28
  • 作者简介:苏俊（1978—），男，安徽巢湖人，硕士研究生，副教授，主要从事法医病理学研究；E-mail：1344502505@qq.com
  • 基金资助:

    贵州省科学技术基金资助项目（黔科合J字LKZ [2010] 13号）

Serum IgE in Guinea Pigs Died of Anaphylactic Shock and Its Forensic Significance When the Guinea Pig Cannot be Timely Refrigerated

SU JUN1, YU JIAN2, LIU YONG2   

  1. （1. Department of Pathology, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China; 2. Center of Forensic Science, Affiliated Hospital of Zunyi Medical College, Zunyi 563003, China）
  • Online:2015-10-25 Published:2015-10-28

被引次数

1

摘要： 目的 研究豚鼠过敏性猝死后血清IgE随死亡时间（PMI）的变化规律，探讨冷藏尸体血清IgE在过敏性猝死法医学鉴定中的价值。 方法 建立过敏性猝死的豚鼠模型，置室温（20 ℃）保存6 h后，于4 ℃冷藏，于死后6、12、24、36和48 h取血清，同时设立对照组。采用酶联免疫吸附试验检测豚鼠血清IgE的含量。 结果 过敏性猝死豚鼠与对照组豚鼠血清IgE含量相比较，差异有统计学意义（P<0.05）；死后6、12、24、36和48 h之间血清IgE含量相比较，差异无统计学意义（P>0.05）。 结论 过敏性猝死6 h后将尸体置于4 ℃冷藏条件下保存，48 h内进行血清IgE检测对过敏性猝死的法医学鉴定仍具有参考价值。

关键词: 法医病理学, 过敏反应, 免疫球蛋白E, 冷藏, 豚鼠

Abstract: Objective To investigate the serum IgE with various postmortem intervals （PMI） in guinea pigs due to sudden death from anaphylactic shock and to explore the effect of refrigeration of corpse on serum IgE level and its application value in forensic medicine. Methods The animal death models of anaphylactic shock were established. The corpses were preserved at room temperature （20 ℃） for 6 h and then refrigerated at 4 ℃. The serum was sampled at 6, 12, 24, 36 and 48 hours after death. The IgE level of serum was detected with ELISA. The control group was also established. Results The serum IgE level had significant difference between the experimental group and the control group （P<0.05）. There was no significant difference among the experimental groups at 6, 12, 24, 36 and 48 hours postmortem （P>0.05）. Conclusion If the corpses were placed in 4 ℃ conditions 6 hours after anaphylactic death, the serum IgE still shows a good marker within 48 h for forensic investigation.

Key words: forensic pathology, anaphylaxis, immunoglobulin E, cold-storage, guinea pigs

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