法医学杂志

法医学杂志 ›› 2020, Vol. 36 ›› Issue (2): 199-203.DOI: 10.12116/j.issn.1004-5619.2020.02.010

• 论著 • 上一篇    下一篇

硬脑膜厚度及生物力学参数时序性变化在死亡时间推断中的应用

李明杰， 王杰， 夏冰， 李竹， 刘江金， 张桥军， 汪家文， 万昌武   

  1. 贵州医科大学法医学院，贵州 贵阳 550004
  • 发布日期:2020-04-25 出版日期:2020-04-28
  • 通讯作者: 汪家文，男，博士，副教授，硕士研究生导师，主要从事法医病理学研究；E-mail：wjwwfs@126.com 万昌武，男，硕士，教授，硕士研究生导师，主要从事法医病理学研究；E-mail：WCW005@sina.com
  • 作者简介:李明杰（1992—），男，硕士研究生，主要从事法医病理学研究；E-mail：lmj0007@126.com
  • 基金资助:
    国家自然科学基金资助项目（81660309）；贵州省科技厅基金资助项目（黔科合平台人才[2018] 5779-3）；贵州医科大学博士启动基金资助项目［院博合J字（2015） 010］

Application of Sequential Changes of Dural Thickness and Biomechanical Parameters in Postmortem Interval Estimation

LI Ming-jie, WANG Jie, XIA Bing, LI Zhu, LIU Jiang-jin, ZHANG Qiao-jun, WANG Jia-wen, WAN Chang-wu   

  1. School of Forensic Medicine, Guizhou Medical University, Guiyang 550004, China
  • Online:2020-04-25 Published:2020-04-28

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摘要： 目的 研究人尸体硬脑膜厚度以及生物力学参数与死亡时间（postmortem interval，PMI）的关系，探讨其用于推断PMI的可行性。 方法 收集尸体检验的硬脑膜样本并按死后6 h、12 h、18 h、24 h、30 h、36 h、48 h、60 h、72 h、84 h、96 h分组，统一制作成4.0 cm×1.0 cm的A、B两块试件。测量A试件厚度后检测极限载荷、最大力变形、抗拉强度、弹性模量、断裂力等生物力学参数，分别拟合厚度和生物力学参数值与PMI的回归方程，并通过验证组验证PMI预测值与实际值之间的差异。B试件经10%中性甲醛溶液固定后制备组织切片观察其形态学变化。 结果 死后6~96 h，硬脑膜厚度逐渐减小，胶原纤维从排列清晰逐渐变为相互融合，细胞核数量逐渐变少；硬脑膜厚度、极限载荷、抗拉强度、弹性模量、断裂力呈时序性下降趋势，其中硬脑膜厚度、极限载荷、弹性模量、断裂力与PMI具有相关性（P<0.05）。回代检验中验证组PMI实际值和预测值之间差异无统计学意义（P>0.05）。 结论 人死后6~96 h的硬脑膜厚度、极限载荷、弹性模量、断裂力呈时序性变化，利用其与PMI的关系建立的回归方程可用于PMI推断。

关键词: 法医病理学, 生物力学, 硬脑膜, 死亡时间

Abstract: Objective To study the relations of the dural thickness and the biomechanical parameters with postmortem interval （PMI） of human cadavers, and to explore the feasibility of the two indexes used for PMI estimation. Methods Dural samples were collected at different postmortem intervals of 6 h, 12 h, 18 h, 24 h, 30 h, 36 h, 48 h, 60 h, 72 h, 84 h and 96 h, then fabricated into 4.0 cm×1.0 cm A and B test specimens. The thickness of the A test specimen was measured, and the biomechanical parameters, such as ultimate load, maximum force deformation, tensile strength, elastic modulus and fracture force, were measured. The regression equations of thickness, biomechanical parameters and PMI were fitted respectively, and the difference between the predicted value and actual measured value of PMI was verified by the verification group. The B test specimen was fixed with 10% neutral formaldehyde solution, then tissue sections were prepared to observe its morphological changes. Results From 6 h to 96 h after death, the dural thickness decreased gradually, the collagen fibers gradually changed from clear arrangement to mutual fusion, and the number of nuclei decreased gradually. The dural thickness, ultimate load, tensile strength, elastic modulus and fracture force decreased sequentially, among which the dural thickness, ultimate load, elastic modulus and fracture force had a correlation with PMI （P<0.05）. In the return test, the difference between the predicted value and actual measured value of PMI in the verification group had no statistical significance （P>0.05）. Conclusion The dural thickness, ultimate load, elastic modulus and fracture force change sequentially from 6 h to 96 h after death. The regression equation established by the relationship between the changes and PMI can be used for PMI estimation.

Key words: forensic pathology, biomechanics, cerebral dura mater, postmortem interval