法医学杂志

法医学杂志 ›› 2021, Vol. 37 ›› Issue (1): 7-10.DOI: 10.12116/j.issn.1004-5619.2020.400207

• 论 著 • 上一篇    下一篇

大鼠死后肌肉总腺苷酸及腺苷酸能荷的时序性变化

朱伟豪1 ，郑哲2 ，孙凯1 ，杨梦姿1 ，钱茂升1 ，莫耀南1   

  1. 1. 河南科技大学法医学院，河南 洛阳 471003；2. 重庆医科大学基础医学院法医学系，重庆 400016
  • 收稿日期:2020-02-16 发布日期:2021-02-25 出版日期:2021-02-28
  • 通讯作者: 莫耀南，男，教授，博士，主要从事法医学研究和教学；E-mail：moyn63@sina.com
  • 作者简介:朱伟豪（1993—），男，硕士研究生，主要从事法医病理学研究；E-mail：1243651854@qq.com
  • 基金资助:
    河南科技大学研究生创新基金资助项目（CXJJ-2019-KJ21）

Sequential Changes of Total Adenine Nucleotide and AdenylicAcid Energy Charge in Muscles of Rats after Death

ZHU Wei-hao1 , ZHENG Zhe2 , SUN Kai1 , YANG Meng-zi1 , QIAN Mao-sheng1 , MO Yao-nan1   

  1. 1. School of Forensic Medicine, Henan University of Science and Technology, Luoyang 471003, Henan Province, China; 2. Department of Forensic Medical, College of Basic Medicine, Chongqing Medical Univer? sity, Chongqing 400016, China
  • Received:2020-02-16 Online:2021-02-25 Published:2021-02-28

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摘要： 目的 测定大鼠死后不同时间双后肢肌肉嘌呤腺苷酸［三磷酸腺苷（adenosine triphosphate，ATP）、 二磷酸腺苷（adenosine diphosphate，ADP）、一磷酸腺苷（adenosine monophosphate，AMP）］含量，计算总腺苷 酸（total adenine nucleotide，TAN）含量和腺苷酸能荷（adenylic-acid energy charge，AEC）的变化值并探究 其与死亡时间（postmortem interval，PMI）的关系。 方法 健康大鼠颈部脱臼处死后，20 ℃恒温保存，分别于 死后 0、24、48、72、96、120、144、168 h 提取大鼠双后肢肌肉，利用反相高效液相色谱法测定嘌呤腺苷酸含 量，计算双后肢肌肉组织的TAN和AEC，并分别建立其与PMI关系的相关回归方程。 结果 大鼠死亡168 h 内，ATP、ADP变化趋势不同，AMP的含量持续增加；TAN值随PMI的延长而逐渐增加，而AEC在死后168 h 内，整体呈现下降趋势。AEC随PMI变化规律明显，决定系数较高（R2 为0.903），曲线拟合关系较好；ATP、 ADP、AMP、TAN与PMI拟合关系不佳（R2 为0.198~0.754）。 结论 AEC的死后变化规律为法医学PMI推断 提供了新的研究思路。

关键词: 法医病理学, 死亡时间, 总腺苷酸, 腺苷酸能荷, 肌肉, 大鼠

Abstract: Objective To determine the purine adenylate [adenosine triphosphate （ATP）, adenosine diphosphate （ADP）, adenosine monophosphate （AMP）] content in the muscles of both hind limbs of rats at different postmortem interval（PMI）, calculate the changes in the total adenine nucleotide （TAN） content and the adenylic-acid energy charge（AEC）, and explore their relationship with PMI. Methods Healthy rats were sacrificed by cervical dislocation and kept at 20 ℃. The muscles of their hind limbs were extracted at 0, 24, 48, 72, 96, 120, 144, and 168 h after death. Reversed-phase high performance liquid chromatography was used to determine the content of purine adenylates, the TAN and AEC of the muscles of the both hind limbs were calculated, and the related regression equations of their relationship with PMI were established. Results Within 168 h of death of rats, the trend of ATP change was different from ADP, and the content of AMP continuously increased. The TAN value gradually increased with the extension of PMI, and the AEC showed a downward trend within 168 h after death. Among them, the patterns of AEC changes with PMI were obvious, the correlation coefficient was high （R2 =0.903）, and the curve fitting relationship was good; the fitting relationship between ATP, ADP, AMP, TAN and PMI was poor （R2 =0.198-0.754）. Conclusion The postmortem change patterns of AEC provide new research ideas for PMI estimation in the forensic field.

Key words: forensic pathology, postmortem interval, total adenine nucleotide, adenylic-acid energy charge, muscle, rats

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