法医学杂志

法医学杂志 ›› 2010, Vol. 26 ›› Issue (4): 282-284.DOI: 10.3969/j.issn.1004-5619.2010.04.013

• 技术与应用 • 上一篇    下一篇

3个miniSTR基因座荧光标记复合扩增体系的建立及应用

杜  冰1，江继平2，杜  宏2，张  林3   

  1. （1． 川北医学院 法医系，四川 南充 637000； 2． 四川省公安厅 刑事侦察局技术处，四川 成都 610041； 3． 四川大学 华西基础医学与法医学院法医物证教研室，四川 成都 610041）
  • 发布日期:2010-08-25 出版日期:2010-08-28
  • 通讯作者: 通信作者：张林，男，教授，博士研究生导师，主要从事法医遗传学研究；E－mail：kjc@scu.edu.cn
  • 作者简介:杜冰（1968—），女，四川南充人，副教授，博士，主要从事法医遗传学研究；E－mail：dbing04@163.com

Construction and Application of Fluorescence Labeled Multiplex Typing System for 3 New miniSTR Loci

DU BING1, JIANG JI-PING2, DU HONG2, ZHANG LIN3   

  1. （1. Department of Forensic Science, North Sichuan Medical College, Nanchong 637000, China; 2. Department of Technique, Criminal Investigation Bureau, Sichuan Public Security Bureau, Chengdu 610041, China; 3. Department of Forensic Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China）
  • Online:2010-08-25 Published:2010-08-28

被引次数

19

摘要： 目的 建立扩增片段小于115 bp，包括D1S1676、D6S1274和D17S1299 3个非CODIS系统的miniSTR基因座复合扩增系统，用于高度降解DNA样本的基因分型。 方法 采用不同荧光染料标记引物，通过PCR扩增，利用310遗传分析仪对100份成都汉族健康无关个体血样以及2份高度降解检材进行检测。 结果 荧光标记复合扩增D1S1676、D6S1274和D17S1299 3个miniSTR基因座，每个基因座均获得了清晰的基因型分型结果。100份样本，3个miniSTR基因座分别检出个9、9、7个等位基因和27、23、18种基因型，基因型分布均符合Hardy-Weinberg平衡。3个基因座在成都汉族人群的累积非父排除率、累积个体识别能力分别为0.999 1和0.916 0。 结论 本系统可以应用于个体识别和亲权鉴定，为DNA高度降解样本分型提供了新的方法。

关键词: 法医遗传学, 多态现象,遗传, 核酸扩增技术, miniSTR, D1S1676, D6S1274, D17S1299

Abstract: Objective To establish a miniSTR multiplex set including three STR loci unlinked from the CODIS loci： D1S1676, D6S1274 and D17S1299, to generate amplified fragment less than 115 bp in size and to study the genotype of degraded DNA samples. Methods After amplification with different fluorescence labeled primers, the amplified products from 100 unrelated individual and  2 highly degraded specimens were analyzed by 310 Genetic Analyzer. Results Three miniSTR loci were determined by fluorescence-labeled multiplex-PCR technique. Each locus was successfully genotyped in all 100 samples. In D1S1676, D6S1274 and D17S1299 loci, 9, 9, 7 alleles and 27, 23, 18 genotypes were observed respectively. The distribution of genotype for three miniSTR loci in Chengdu Han population was in accordance with Hardy-Weinberg equilibrium. The combined exclusion probability and the combined discrimination power of the three STR loci in Chengdu Han population were 0.999 1 and 0.916 0  respectively. Conclusion This miniSTR multiplex set could be used in individual identification and paternity test.  It also provides a new method in the analysis of degraded DNA sample.

Key words: forensic genetics, polymorphism, genetic, nucleic acid amplification techniques, miniSTR, D1S1676, D6S1274, D17S1299

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